Anti-KAP1抗体
参阅全部 KAP1 一抗
兔多克隆抗体to KAP1
Rabbit
适用于: WB, IP, IHC-Pmore details
与反应: Mouse, Human
预测可用于: Rat
Synthetic peptide corresponding to Human KAP1 aa 650-750.
Database link: Q13263
WB: HeLa, 293T, 3T3 whole cell lysate. IP: HeLa whole cell lysate. IHC-P: Human ovarian carcinoma, mouse plasmacytoma, mouse coronal brain tissue.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
浓度
50 µg 浓度为 1 mg/ml
Immunogen affinity purified
Antibodies were affinity purified using the peptide immobilized on solid support.
多克隆
IgG
Abpromise™承诺保证使用ab10484于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (3) | 1/500 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 100 kDa). |
| IP | (1) | Use at 2-10 µg/mg of lysate. |
| IHC-P | (1) | Use a concentration of 2 µg/ml. |
Entrez Gene: 10155 Human
Entrez Gene: 21849 Mouse
Omim: 601742 Human
SwissProt: Q13263 Human
SwissProt: Q62318 Mouse
Unigene: 467408 Human
Unigene: 15701 Mouse
Unigene: 398345 Mouse
Unigene: 198494 Rat
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Western blot - Anti-KAP1 antibody (ab10484)
All lanes : Anti-KAP1 antibody (ab10484) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : 3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 100 kDa
Exposure time: 1 second
Cells prepared using NETN lysis buffer. Chemiluminescence detection.
Immunoprecipitation - Anti-KAP1 antibody (ab10484)
CTCF was immunoprecipitated from 1 mg HeLa whole cell lysate with ab10484 at 6 µg per reaction. Western blot was performed on the immunoprecipitate using ab10484 at 1 µg/mL.
Lysates prepared using NETN lysis buffer.
Lane 1: ab10484 IP in HeLa whole cell lysate.
Lane 2: Contol IgG in HeLa whole cell lysate.
Detection: Chemiluminescence.
Exposure time: 3 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 antibody (ab10484)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma labeling KAP1 with ab10484 at 1/5000 dilution (0.2 μg/ml). DAB detection, Hematoxylin counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 antibody (ab10484)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse plasmacytoma, labeling KAP1 with ab10484 at 1 µg/mL. Detection: DAB.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 antibody (ab10484)Image courtesy of an anonymous Abreview.
ab10484 staining KAP1 in murine coronal brain tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with paraformaldehyde and permeabilized using 1% Triton. Samples were then blocked with 5% serum for 1 hour at 25°C followed by incubation with the primary antibody at 2µg/ml for 16 hours at 4°C. An Alexa-Fluor 488-conjugated donkey anti-rabbit polyclonal was used as secondary antibody at a 1/1000 dilution.
Dentate gyrus of the hippocampus immunostained with DAPI (top) and KAP1 (bottom), which nicely labels the nuclei of the entire dentate gyrus.
