Anti-BACE1抗体
参阅全部 BACE1 一抗
兔多克隆抗体to BACE1
Rabbit
Detects transfected beta-secretase 1.
适用于: WB, ICC/IFmore details
与反应: Mouse, Human
预测可用于: Guinea pig, Cow
Synthetic peptide corresponding to Human BACE1 aa 485-501 (C terminal).
Sequence:
CLRQQHDDFADDISLLK
(Peptide available as ab7883)
WB: U87-MG, HeLa and Mouse brain cell lysate. ICC/IF: SH-SY5Y cells, Neuro-2a cells, HeLa cells.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA
浓度
100 µg 浓度为 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab10716于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/1000. Predicted molecular weight: 56 kDa. | |
| ICC/IF | 1/20 - 1/200. |
Entrez Gene: 23621 Human
Entrez Gene: 23821 Mouse
Omim: 604252 Human
SwissProt: P56817 Human
SwissProt: P56818 Mouse
Unigene: 504003 Human
Unigene: 24044 Mouse
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Western blot - Anti-BACE1 antibody (ab10716)
All lanes : Anti-BACE1 antibody (ab10716) at 1/1000 dilution
Lane 1 : U87-MG cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Mouse brain cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP-conjugated secondary antibody
Predicted band size: 56 kDa
Observed band size: 42,56,70 kDawhy is the actual band size different from the predicted?
Western blot analysis of BACE1 was performed by loading Samples onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with primary antibody overnight at 4°C, washed in TBST, and probed with secondary antibody for 1 hour at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate.
Immunocytochemistry/ Immunofluorescence - Anti-BACE1 antibody (ab10716)
Immunocytochemistry/Immunofluorescent analysis of BACE1 (green) showing staining in the Golgi apparatus of SH-SY5Y cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a10716 in 3% BSA-PBS at a dilution of 1/100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
Immunocytochemistry/ Immunofluorescence - Anti-BACE1 antibody (ab10716)
Immunocytochemistry/Immunofluorescent analysis of BACE1 (green) showing staining in the Golgi apparatus of Neuro-2a cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab10716 in 3% BSA-PBS at a dilution of 1/100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
Immunocytochemistry/ Immunofluorescence - Anti-BACE1 antibody (ab10716)
Immunocytochemistry/Immunofluorescent analysis of BACE1 (green) showing staining in the Golgi apparatus of HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab10716 in 3% BSA-PBS at a dilution of 1/100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
