Anti-CSNK2A1抗体
参阅全部 CSNK2A1 一抗
兔多克隆抗体to CSNK2A1
Rabbit
Reacts with alpha subunit.
适用于: WBmore details
与反应: Human
预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
Synthetic peptide corresponding to Human CSNK2A1 aa 1-100.
Database link: 1457
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
浓度
50 µg 浓度为 1 mg/ml
Immunogen affinity purified
Antibodies were affinity purified using the peptide immobilized on solid support.
多克隆
IgG
Abpromise™承诺保证使用ab10466于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (3) | 1/1000 - 1/5000. Detects a band of approximately 42 kDa (predicted molecular weight: 45 kDa). |
Entrez Gene: 432370 Chicken
Entrez Gene: 1457 Human
Entrez Gene: 12995 Mouse
Entrez Gene: 399319 Xenopus laevis
Omim: 115440 Human
SwissProt: P21868 Chicken
SwissProt: P68400 Human
SwissProt: Q60737 Mouse
SwissProt: P33674 Rabbit
SwissProt: P28020 Xenopus laevis
Unigene: 644056 Human
Unigene: 654675 Human
Unigene: 23692 Mouse
Unigene: 4231 Rat
Unigene: 11560 Xenopus laevis
Casein kinase 2 alpha 1 polypeptide antibody
Casein kinase 2 alpha antibody
Casein kinase II alpha 1 antibody
Casein kinase II alpha 1 subunit antibody
Casein kinase II alpha subunit antibody
Casein kinase II subunit alpha antibody
CK II alpha antibody
CK II antibody
CK2 alpha antibody
CK2 catalytic subunit alpha antibody
CK2A1 antibody
CKII antibody
CKIIalpha antibody
CSK21_HUMAN antibody
CSNK2A1 antibody
Western blot - Anti-CSNK2A1 antibody (ab10466)
Western blot - Anti-CSNK2A1 antibody (ab10466)
Western blot - Anti-CSNK2A1 antibody (ab10466)
All lanes : Anti-CSNK2A1 antibody (ab10466) at 1/2500 dilution
Lane 1 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 45 kDa
Western blot - Anti-CSNK2A1 antibody (ab10466)This image is courtesy of an Abreview submitted by Svetlana Khoronenkova
All lanes : Anti-CSNK2A1 antibody (ab10466) at 1/5000 dilution (LI-COR Blocker (1:1) in PBS + 0.1% Tween for 3 hours)
Lane 1 : Whole cell lysate of HeLa cells
Lane 2 : siRNA to CK2 alpha in HeLa cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : An Alexa Fluor®680-conjugated Goat anti-rabit polyclonal at 1/10000 dilution
Predicted band size: 45 kDa
Observed band size: 42 kDawhy is the actual band size different from the predicted?
Blocking Step: 50% LI-COR® Odyssey® Blocking Buffer for 45 minutes at room temperature.
All non-specific bands were very weak.
