Anti-KAT13C / NCOA2抗体
参阅全部 KAT13C / NCOA2 一抗
兔多克隆抗体to KAT13C / NCOA2
Rabbit
We have data to indicate that this antibody may not cross react with Xenopus laevis. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
适用于: WB, IHC-P, IPmore details
与反应: Mouse, Human
预测可用于: Rat, Rabbit, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Gorilla, Chinese hamster
Synthetic peptide corresponding to Human KAT13C/ NCOA2 aa 700-800.
Database link: Q15596
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
浓度
50 µg 浓度为 1 mg/ml
Immunogen affinity purified
Antibodies were affinity purified using the peptide immobilized on solid support.
多克隆
IgG
Abpromise™承诺保证使用ab10491于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (3) | 1/1000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 175 kDa). |
| IHC-P | Use at an assay dependent concentration. | |
| IP | (1) | Use 6µg for 10 cells. |
Entrez Gene: 10499 Human
Entrez Gene: 17978 Mouse
Omim: 601993 Human
SwissProt: Q15596 Human
SwissProt: Q61026 Mouse
Unigene: 446678 Human
Unigene: 2537 Mouse
Unigene: 49136 Rat
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Western blot - Anti-KAT13C / NCOA2 antibody (ab10491)
All lanes : Anti-KAT13C / NCOA2 antibody (ab10491) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : NCOA2 knockout HEK-293T cell lysate
Lane 3 : PC-3 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 175 kDa
Observed band size: 160 kDawhy is the actual band size different from the predicted?
False colour image of Western blot: Anti-KAT13C / NCOA2 antibody staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab10491 was shown to bind specifically to KAT13C / NCOA2. A band was observed at 160 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in NCOA2 knockout cell line ab265068 (knockout cell lysate ab258530). To generate this image, wild-type and NCOA2 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
Immunoprecipitation - Anti-KAT13C / NCOA2 antibody (ab10491)
ab10491 used for WB at 0.1 µg/ml (A)
Lane 1: Whole cell lysate Hela, 50 ug
Lane 2: Whole cell lysate Hela, 15 ug
Lane 3: Whole cell lysate HEK293T, 50 ug
Lane 4: Whole cell lysate Jurkat, 50 ug
ab10491 was used at 1 µg/ml for WB and used for IP at 6 µg/mg lysate (B)
Lane 1-3: ab10491]
Lane4: Control IgG
Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).
Western blot - Anti-KAT13C / NCOA2 antibody (ab10491)
All lanes : Anti-KAT13C / NCOA2 antibody (ab10491) at 1 µg/ml
Lane 1 : TCMK-1 cells
Lane 2 : 4T1 cells
Lane 3 : CT26.WT cells
Lysates/proteins at 50 µg per lane.
Predicted band size: 175 kDa
