| 存储条件 | -20℃ |
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Human IL-1 R4 ELISA Kit (ST2) is a Sandwich (quantitative) ELISA for the measurement of Human IL-1 R4 (ST2) in Human in Biofluids, Cell Culture Media samples.
隐藏别名
DER4, ST2, T1, IL1RL1, Interleukin-1 receptor-like 1, Protein ST2
Colorimetric
Plasma, Cell culture supernatant, Serum
Human
Sandwich (quantitative)
< 2 pg/mL
1.65 - 1200 pg/mL
5h
Microplate
Human IL-1 R4 ELISA Kit (ST2) (ab100563) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-1 R4 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human IL-1 R4 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 R4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-1 R4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 R4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
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Representative standard curve using ab100563
Representative standard curve using ab100563
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