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Human EGF ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human EGF in Human in Biofluids, Cell Culture Media samples.
隐藏别名
Pro-epidermal growth factor, EGF
Colorimetric
Plasma, Cell culture supernatant, Serum
Human
Sandwich (quantitative)
< 1 pg/mL
0.82 - 200 pg/mL
Microplate
Abcam's EGF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human EGF in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human EGF coated on a 96-well plate. Standards and samples are pipetted into the wells and EGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human EGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of EGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimisation may be required with urine samples.
Standard curve in Diluent B : mean of duplicates (+/-SD)
Standard curve in Diluent A : mean of duplicates (+/-SD)
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