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Human MMP3 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human MMP3 in Human in Biofluids, Cell Culture Media samples.
隐藏别名
STMY1, MMP3, Stromelysin-1, SL-1, Matrix metalloproteinase-3, Transin-1, MMP-3
Colorimetric
Plasma, Cell culture supernatant, Serum
Human
Sandwich (quantitative)
< 0.3 ng/mL
0.41 - 100 ng/mL
Microplate
Abcam's MMP3 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MMP3 pro and active forms in serum, plasma, and cell culture supernatants. (Collect plasma using heparin as an anticoagulant. EDTA and Citrate are not recommended).
This assay employs an antibody specific for Human MMP3 coated on a 96- well plate. Standards and samples are pipetted into the wells and MMP3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MMP3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get results in 90 minutes with Human MMP3 ELISA Kit (ab189572) from our SimpleStep ELISA® range.
Optimisation may be required with urine samples.
Blue Ice
-20°C
-20°C
-20°C
Representative Standard Curve using ab100607.
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