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Human TNFRSF18 ELISA Kit (GITR) is a Sandwich (quantitative) ELISA for the measurement of Human TNFRSF18 (GITR) in Human in Biofluids, Cell Culture Media samples.
隐藏别名
CD357, AITR, GITR, UNQ319/PRO364, TNFRSF18, Tumor necrosis factor receptor superfamily member 18, Activation-inducible TNFR family receptor, Glucocorticoid-induced TNFR-related protein
Colorimetric
Plasma, Cell culture supernatant, Serum
Human
Sandwich (quantitative)
< 15 pg/mL
13.7 - 10000 pg/mL
Microplate
Abcam's TNFRSF18 (GITR) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TNFRSF18 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human TNFRSF18 coated on a 96-well plate. Standards and samples are pipetted into the wells and TNFRSF18 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TNFRSF18 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TNFRSF18 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples
Blue Ice
-20°C
-20°C
-20°C
Standard curve in diluent A, with background signal subtracted (duplicates; +/- SD).
GITR measured in PBMC lysates (RIPA; 0.02-0.1 mg of proteins tested per well) after 72 hours of culture (1e6 cells per mL) in 0.1% DMSA (CTRL), 5 ug x mL-1 PHA (Sigma) or in 50ng x mL-1 PMA (ab120297) with 1uM ionomycin (ab120116) (P/I) (duplicates +/- SD).
Representative standard curve using ab100527
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