| 存储条件 | -20℃ |
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Human Osteopontin ELISA Kit is a sandwich ELISA designed to quantify Human Osteopontin with a sensitivity of 50 pg/mL.
- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 74.07 - 18000 pg/mL
- Cited in over 10 publications
隐藏别名
BNSP, OPN, PSEC0156, SPP1, Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, Urinary stone protein, Uropontin, SPP-1
Colorimetric
Urine, Plasma, Cell culture supernatant, Milk, Serum
Human
Sandwich (quantitative)
< 50 pg/mL
74.07 - 18000 pg/mL
Microplate
Human Osteopontin ELISA Kit ab100618 is a sandwich ELISA to measure Human Osteopontin in serum, plasma, cell culture supernatant, urine, milk with a sensitivity of 50 pg/ml.
How the assay works
This assay employs an antibody specific for Human Osteopontin coated on a 96-well plate. Standards and samples are pipetted into the wells and Osteopontin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Osteopontin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Osteopontin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Human Osteopontin ELISA Kit ab100618 protocol summary
1. Add standard or sample to each well used. Incubate at room temperature
2. Wash and add prepared biotinylated antibody to each well. Incubate at room temperature.
3. Wash and add prepared streptavidin solution. Incubate at room temperature.
4. Add TMB Development Solution to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read at 450nm immediately.
Get results in 90 minutes with our SimpleStep ELISA® kit
Human Osteopontin ELISA Kit (ab192143)
Optimization may be required with urine samples
Blue Ice
-20°C
-20°C
-20°C
Representative Standard Curve using ab100618.
Representative Standard Curve using ab100618.
Osteopontin measured in biological fluids showing quantity (pg) per mL of tested sample. Human serum diluted 10-30 fold. Human plasma diluted 50 fold - below detection. Human milk diluted 18000-54000 fold. Human urine diluted 100 fold.
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