Human IL-1 alpha ELISA Kit

Human IL-1 alpha ELISA Kit ab100560 is a sandwich ELISA to measure Human IL-1 alpha in serum, plasma, cell culture supernatant with a sensitivity of 2 pg/ml.

How the assay works
This assay employs an antibody specific for Human IL-1 alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-1 alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm

Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Human IL-1 alpha ELISA Kit ab100560 protocol summary
1. Add standard or sample to each well used. Incubate at room temperature
2. Wash and add prepared biotinylated antibody to each well. Incubate at room temperature.
3. Wash and add prepared streptavidin solution. Incubate at room temperature.
4. Add TMB Development Solution to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read at 450nm immediately.

Get results in 90 minutes with our SimpleStep ELISA® kit
Human IL-1a ELISA Kit (ab178008)

Optimization may be required with urine samples.