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Human IL-10 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human IL-10 in Human in Biofluids, Cell Culture Media samples.
隐藏别名
Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, IL10
Colorimetric
Plasma, Cell culture supernatant, Serum
Human
Sandwich (quantitative)
< 1 pg/mL
2.34 - 150 pg/mL
Microplate
Abcam's IL-10 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-10 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human IL-10 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get results in 90 minutes with Human IL-10 ELISA Kit (ab185986) from our SimpleStep ELISA® range.
Produced using a non-baculovirus expression system.
Optimization may be required with urine samples
Blue Ice
-20°C
-20°C
-20°C
Typical Standard Curve.
Representative standard curve using ab100549
IL-10 measured in culture supernatants from control (-) or activated cell lines (dilution range 1/1-1/00), stimulated for 24 hours with 50 ng x mL-1 of PMA (P)(ab120297) and with the addition of 1 ug x mL-1 of LPS (Sigma) (P/L) for the last 6 hours or in PBMC supernatants (dilution range 1/10-1/1000), from control or treated cells ((PHA) 48 hours with 2% PHA-M; LifeTechnologies) (duplicates +/- SD).
Standard curve (assay diluent B) with background signal subtracted (duplicates; +/- SD).
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