| 存储条件 | -20℃ |
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Human IL-10RB ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human IL-10RB in Human in Biofluids, Cell Culture Media samples.
隐藏别名
CDw210b, CRFB4, D21S58, D21S66, IL10RB, Interleukin-10 receptor subunit beta, IL-10 receptor subunit beta, IL-10R subunit beta, IL-10RB, Cytokine receptor class-II member 4, Cytokine receptor family 2 member 4, Interleukin-10 receptor subunit 2, CRF2-4, IL-10R subunit 2, IL-10R2
Colorimetric
Plasma, Cell culture supernatant, Serum
Human
Sandwich (quantitative)
< 5 pg/mL
4.12 - 3000 pg/mL
Microplate
Abcam's IL-10RB Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme linked immunosorbent assay for the quantitative measurement of Human IL-10RB in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human IL-10RB coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-10RB present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-10RB antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-10RB bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples
Blue Ice
-20°C
-20°C
-20°C
Representative standard curve using ab100550
Representative standard curve using ab100550
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