Anti-muscle Actin antibody [HHF35]

• 产品名称

  Anti-muscle Actin抗体[HHF35]
  参阅全部 muscle Actin 一抗

• 描述

  小鼠单克隆抗体[HHF35] to muscle Actin

• 宿主

  Mouse

• 特异性

  This antibody recognizes muscle specific alpha and gamma actin isomers but is non-reactive with beta isomers.

• 经测试应用

  适用于: IHC-Pmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Full length native protein (purified) corresponding to Human muscle Actin. The SDS extracted protein fraction of human myocardium.
  Database link: P68133

• 常规说明

  This antibody stains myocardial, skeletal muscle and smooth muscle cells as well as myoepithelial cells, pericytes of small vessels. All other non-muscle cells were negative.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

• 存储溶液

  pH: 7.3
  Preservative: 0.1% Sodium azide

• 纯度

  Tissue culture supernatant

• Primary antibody说明

  This antibody stains myocardial, skeletal muscle and smooth muscle cells as well as myoepithelial cells, pericytes of small vessels. All other non-muscle cells were negative.

• 克隆

  单克隆

• 克隆编号

  HHF35

• 同种型

  IgG1

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab7813于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 58 Human

• Omim: 102610 Human

• SwissProt: P68133 Human

• Unigene: 1288 Human

• 别名

• ACTA2 antibody

• ACTC antibody

• ACTC1 antibody

• Actin antibody

• ACTS_HUMAN antibody

• ACTSA antibody

• Alpha 2 actin antibody

• alpha skeletal muscle antibody

• Alpha-actin-1 antibody

• Cardiac muscle alpha actin 1 antibody

• Skeletal muscle alpha actin 1 antibody

• a actin antibody

• ACTA antibody

• ACTA1 antibody

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-muscle Actin antibody [HHF35] (ab7813)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle labelling muscle Actin with ab7813.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-muscle Actin antibody [HHF35] (ab7813)

  Ab7813 staining human normal skeletal muscle. Staining is localized to the cytoplasm.
  Left panel: with primary antibody diluted 1:4000. Right panel: isotype control.
  Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.