Anti-CX3CR1抗体
参阅全部 CX3CR1 一抗
兔多克隆抗体to CX3CR1
Rabbit
适用于: IHC-P, WBmore details
不适用于: ICC/IF
与反应: Human
预测可用于: Mouse, Rat
Synthetic peptide, corresponding to amino acids 175-189 of Human CX3CR 1.
THP-1 cell lysate
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at +4°C.
pH: 7.2
Preservative: 0.02% Sodium azide
Affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab8020于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (1) | Use a concentration of 10 µg/ml. |
| WB | (3) | Use a concentration of 1 µg/ml. Predicted molecular weight: 50 kDa. |
应用说明
Is unsuitable for ICC/IF.
Entrez Gene: 1524 Human
Entrez Gene: 13051 Mouse
Omim: 601470 Human
SwissProt: P49238 Human
SwissProt: Q9Z0D9 Mouse
Unigene: 78913 Human
Unigene: 44065 Mouse
Unigene: 10482 Rat
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Western blot - Anti-CX3CR1 antibody (ab8020)
All lanes : Anti-CX3CR1 antibody (ab8020) at 1 µg/ml
Lane 1 : Wild-type THP-1 cell lysate
Lane 2 : CX3CR1 knockout THP-1 cell lysate
Lane 3 : Human Spleen cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : Caco-2 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 42 kDawhy is the actual band size different from the predicted?
False colour image of Western blot: Anti-CX3CR1 antibody staining at 1 ug/ml, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab8020 was shown to bind specifically to CX3CR1. A band was observed at 42 kDa in wild-type THP-1 cell lysates with no signal observed at this size in CX3CR1 knockout cell line ab273713 (knockout cell lysate ab275510). To generate this image, wild-type and CX3CR1 knockout THP-1 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CX3CR1 antibody (ab8020)
ab8020 at 10µg/ml staining CX3CR1 in human spleen cells by IHC
Western blot - Anti-CX3CR1 antibody (ab8020)
All lanes : Anti-CX3CR1 antibody (ab8020) at 1/500 dilution
Lane 1 : THP-1 cell lysate with absence of blocking peptide
Lane 2 : THP-1 cell lysate with presence of blocking peptide
Predicted band size: 50 kDa
Observed band size: 50 kDa
