Anti-Neurofilament heavy polypeptide antibody [NF-01]

• 产品名称

  Anti-Neurofilament heavy polypeptide抗体[NF-01]
  参阅全部 Neurofilament heavy polypeptide 一抗

• 描述

  小鼠单克隆抗体[NF-01] to Neurofilament heavy polypeptide

• 宿主

  Mouse

• 特异性

  This antibody recognizes a phosphorylated epitope on heavy neurofilament protein (210 kDa) of various species.

• 经测试应用

  适用于: IHC-P, Flow Cytmore details

• 种属反应性

  与反应: Human
  预测可用于: a wide range of other species, Mammals

• 免疫原

  Full length native protein (purified) corresponding to Pig Neurofilament heavy polypeptide. A pellet of pig brain cold-stable proteins after depolymerization of microtubules.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.40
  Preservative: 0.097% Sodium azide
  Constituent: PBS

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Protein A purified

• 纯化说明

  >95 % pure (by PAGE)

• 克隆

  单克隆

• 克隆编号

  NF-01

• 同种型

  IgG1

• 轻链类型

  unknown

The Abpromise guarantee

Abpromise™承诺保证使用ab7795于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 4744 Human

• Omim: 162230 Human

• SwissProt: P12036 Human

• Unigene: 198760 Human

• 别名

• Neurofilament heavy polypeptide 200kDa antibody

• Neurofilament heavy polypeptide antibody

• Neurofilament triplet H protein antibody

• NF H antibody

• NF-H antibody

• NFH antibody

• NFH_HUMAN antibody

• 200 kDa neurofilament protein antibody

• CMT2CC antibody

• Nefh antibody

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [NF-01] (ab7795)

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue sections labelling Neurofilament heavy polypeptide protein with ab7795 at 5 µL.

• 

  Flow Cytometry - Anti-Neurofilament heavy polypeptide antibody [NF-01] (ab7795)

  Overlay histogram showing SH-SY5Y cells stained with ab7795 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7795, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was a goat anti-mouse DyLight® 488  (IgG; H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.