Anti-IRAKM抗体
参阅全部 IRAKM 一抗
兔多克隆抗体to IRAKM
Rabbit
Anti-IRAK-M has no cross response to IRAK or IRAK2.
适用于: ICC, IHC-P, WBmore details
与反应: Mouse, Rat
Synthetic peptide:
CSSKFSWDEYEQYKKE
, corresponding to C terminal amino acids 581-596 of Human IRAKM (Peptide available as ab6231.)
Interleukin-1 (IL-1) and lipopolysaccharide (LPS)induces cellular response through IL-1 receptor (IL-1R) and Toll like receptors (TLR). IL-1 receptor associated kinase (IRAK and IRAK2) mediates the activation of NF-kB by IL-1/Toll receptors (Wesche H et al.1999,Muzio M et al.1997), which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in theIRAK/Pelle family was recently identified and designated IRAK-M (Cao Z et al.1996). IRAKs associate with IL-1/Toll receptors after IL-1 or LPS stimulation and thedominant negative mutants of IRAKs inhibit IL-1 orLPS induced NF-kB activation. Members inIRAK/Pelle family play a central role in IL-1R/TLRmediated inflammatory responses to cytokine IL-1and LPS.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
pH: 7.2
Preservative: 0.02% Sodium azide
浓度
100 µg 浓度为 1 mg/ml
Affinity purified
Purified IgG prepared by ion exchange chromatography. IRAK-M Antibody is affinity chromatography purified via peptide column.
Interleukin-1 (IL-1) and lipopolysaccharide (LPS)induces cellular response through IL-1 receptor (IL-1R) and Toll like receptors (TLR). IL-1 receptor associated kinase (IRAK and IRAK2) mediates the activation of NF-kB by IL-1/Toll receptors (Wesche H et al.1999,Muzio M et al.1997), which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in theIRAK/Pelle family was recently identified and designated IRAK-M (Cao Z et al.1996). IRAKs associate with IL-1/Toll receptors after IL-1 or LPS stimulation and thedominant negative mutants of IRAKs inhibit IL-1 orLPS induced NF-kB activation. Members inIRAK/Pelle family play a central role in IL-1R/TLRmediated inflammatory responses to cytokine IL-1and LPS.
多克隆
IgG
Abpromise™承诺保证使用ab8116于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC | Use at an assay dependent concentration. | |
| IHC-P | Use a concentration of 2 µg/ml. | |
| WB | (3) | Use a concentration of 0.5 - 1 µg/ml. |
Entrez Gene: 73914 Mouse
SwissProt: Q8K4B2 Mouse
Unigene: 146194 Mouse
IL1 receptor associated kinase M antibody
Interleukin 1 receptor associated kinase 3 antibody
Interleukin 1 receptor associated kinase M antibody
Interleukin-1 receptor-associated kinase 3 antibody
IRAK M antibody
IRAK-3 antibody
IRAK-M antibody
Irak3 antibody
IRAK3_HUMAN antibody
ASRT5 antibody
IL 1 receptor associated kinase M antibody
IL-1 receptor-associated kinase M antibody
Western blot - Anti-IRAKM antibody (ab8116)
All lanes : Anti-IRAKM antibody (ab8116) at 1 µg/ml
Lane 1 : Mouse spleen tissue lysates
Lane 2 : Rat liver tissue lysate
Observed band size: 68 kDawhy is the actual band size different from the predicted?
Western blot analysis of IRAKM in mouse spleen (M) and rat liver (R) tissue lysates with anti-IRAKM at 1 µg /ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRAKM antibody (ab8116)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of A20 cells staining IRAKM with ab8116 at 20 µg/ml
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRAKM antibody (ab8116)
Immunohistochemical staining of rat liver tissue using IRAK-M antibody at 2 µg/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRAKM antibody (ab8116)
ab8116 at 2µg/ml staining IRAKM in rat liver cells by IHC
Immunocytochemistry - Anti-IRAKM antibody (ab8116)Image from Lyn-Kew K et al, PLoS One. 2010 Jun 16;5(6):e11145, Fig 4.
ab8116 staining IRAKM in murine lung macrophages by Immunocytochemistry/ Immunofluorescence.<.br>
The cells were permeablized with a 1:1 mixture of methanol and acetone. After washing with PBS, ab8116 was applied and the cells incubated for one hour. After washing, antibodies against the primary antibody labeled with FITC were applied to the cells and incubated for one hour. The cell were then mounted in DAPI mounting media and viewed on the fluorescent microscope.
Immunocytochemistry - Anti-IRAKM antibody (ab8116)
Immunofluorescence of IRAK-M in Rat Liver cells using ab8116 at 10 ug/ml.
