Anti-NCAM1 antibody [MEM-188]

• 产品名称

  Anti-NCAM1抗体[MEM-188]
  参阅全部 NCAM1 一抗

• 描述

  小鼠单克隆抗体[MEM-188] to NCAM1

• 宿主

  Mouse

• 特异性

  CD56 antigen (human). This antibody reacts with 180 kDa isoform characteristic for leucocytes. Reactivity with other NCAM isoforms has not been tested. Positive membrane-specific staining was observed in the paraprostatic autonomic ganglion.

• 经测试应用

  适用于: Flow Cytmore details

• 种属反应性

  与反应: Human
  预测可用于: Non human primates

• 免疫原

  Tissue, cells or virus corresponding to Human NCAM1. KG-1 acute myelogenous leukaemia cell line

• 阳性对照

• Flow Cyt: Human peripheral blood.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.40
  Preservative: 0.097% Sodium azide
  Constituent: PBS

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Protein A purified

• 克隆

  单克隆

• 克隆编号

  MEM-188

• 同种型

  IgG2a

The Abpromise guarantee

Abpromise™承诺保证使用ab8233于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 4684 Human

• Omim: 116930 Human

• SwissProt: P13591 Human

• Unigene: 503878 Human

• 别名

• cell adhesion molecule, neural, 1 antibody

• MSK 39 antibody

• MSK39 antibody

• N-CAM-1 antibody

• NCAM 1 antibody

• NCAM antibody

• NCAM C antibody

• NCAM-1 antibody

• NCAM1 antibody

• NCAM1_HUMAN antibody

• NCAMC antibody

• Neural cell adhesion molecule 1 antibody

• Neural cell adhesion molecule NCAM antibody

• OTTHUMP00000235666 antibody

• antigen MSK39 identified by monoclonal antibody 5.1H11 antibody

• antigen recognized by monoclonal antibody 5.1H11 antibody

• CD56 antibody

• 

  Flow Cytometry - Anti-NCAM1 antibody [MEM-188] (ab8233)

  Flow cytometry analysis (surface staining) of human peripheral blood lymphocytes with ab8233 (GAM-APC).

• 

  Flow Cytometry - Anti-NCAM1 antibody [MEM-188] (ab8233)

  Overlay histogram showing SH-SY5Y cells stained with ab8233 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8233, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.