Anti-BrdU antibody [MoBu-1]

• 产品名称

  Anti-BrdU抗体[MoBu-1]
  参阅全部 BrdU 一抗

• 描述

  小鼠单克隆抗体[MoBu-1] to BrdU

• 宿主

  Mouse

• 特异性

  This antibody reacts specifically with BrdU incorporated into DNA during S-phase of a cell cycle. It is useful for detecting proliferating cells by flow cytometry or immunofluorescence staining. The reaction shows a clear, nuclear confined speckled pattern. It reacts also specifically with 5-bromouridine (BrU).

• 经测试应用

  适用于: IHC-P, ICC/IF, Flow Cyt (Intra)more details

• 种属反应性

  与反应: Species independent
  

• 免疫原

  Chemical/ Small Molecule corresponding to BrdU.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C. Do Not Freeze.

• 存储溶液

  pH: 7.40
  Preservative: 0.097% Sodium azide
  Constituent: PBS

• 纯度

  Protein A purified

• 纯化说明

  >95 % (by PAGE).

• 克隆

  单克隆

• 克隆编号

  MoBu-1

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab8039于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 别名

• Bromodeoxyuridine antibody

• BUdr antibody

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [MoBu-1] (ab8039)

  Immunohistochemical analysis of parafin-embedded bromodeoxyuridine-labelled chick embryo cells with (MoBu-1) 5-bromodeoxyuridine 

• 

  Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [MoBu-1] (ab8039)

  ICC/IF image of ab8039 stained HeLa cells, both BrdU treated (left image) and normal cells (right image). The cells were 100% methanol fixed (5 min) and then subjected to acid hydrolysis using 2M HCL in 0.1% PBS-Tween for 30 minutes at room temperature to denature the DNA. They were  then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8039, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. Positive staining can be seen in the BrdU treated cells, but not in the normal cells, demonstrating specificity for BrdU.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [MoBu-1] (ab8039)

  IHC image of ab8039 staining, both in normal and BrdU treated rat liver formalin fixed paraffin embedded tissue sections, performed on a Leica Bond<sup>tm< sup>="" system="" using="" the="" standard="" protocol="" f.="" section="" was="" pre-treated="" heat="" mediated="" antigen="" retrieval="" with="" sodium="" citrate="" buffer="" (ph6,="" epitope="" solution="" 1)="" for="" 20="" mins.="" then="" incubated="" ab8039,="" 5&micro;g="" ml,="" 15="" mins="" at="" room="" temperature="" and="" detected="" an="" hrp="" conjugated="" compact="" polymer="" system.="" dab="" used="" as="" chromogen.="" counterstained="" haematoxylin="" mounted="" dpx.

  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• 

  Flow Cytometry (Intracellular) - Anti-BrdU antibody [MoBu-1] (ab8039)

  Intracellular Flow Cytometry analysis of CEM (human acute lymphoblastic leukemia) cells labelling BrdU with ab8039 at 1µg/mL. Goat anti-mouse IgG was used as the secondary antibody.The individual cell cycle phases (S, G1, G2/M-phase) are indicated on the figure.