Anti-MAD1L1/MAD1抗体[9B10]
参阅全部 MAD1L1/MAD1 一抗
小鼠单克隆抗体[9B10] to MAD1L1/MAD1
Mouse
适用于: Flow Cytmore details
不适用于: WB
与反应: Human
Recombinant full length protein corresponding to Human MAD1L1/MAD1.
Database link: Q9Y6D9, Q13586
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.1% Sodium azide
Constituent: PBS
Protein A purified
单克隆
9B10
IgG2b
Abpromise™承诺保证使用ab5783于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt | Use 0.1µg for 106 cells. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
应用说明
Is unsuitable for WB.
Entrez Gene: 8379 Human
Omim: 602686 Human
SwissProt: Q9Y6D9 Human
Unigene: 654838 Human
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![Flow Cytometry - Anti-MAD1L1/MAD1 antibody [9B10] (ab5783)](https://www.abcam.cn/ps/products/5/ab5783/Images/ab5783-195393-anti-mad1-antibody-9b10-flow-cytometry.jpg)
Flow Cytometry - Anti-MAD1L1/MAD1 antibody [9B10] (ab5783)
Overlay histogram showing HeLa cells stained with ab5783 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab5783, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
