Chemiluminescent HRP底物(membrane)
A 2 component chemiluminescent substrate for the detection of horseradish peroxidase (HRP) in western blotting.
The Abcam Chemiluminescent HRP Substrate (membrane) is very sensitive and great care must be taken to optimize the individual assay components (antibodies, conjugates, solid phase, etc.) to minimize background reactivity associated with nonspecific immunochemical reactivities
Prepare 1x substrate by mixing 1 ml of the chemiluminescent reagent with 2 ml of the chemiluminescent reaction buffer. Mix well and protect from light. More or less of the 1x substrate can be prepared using the same ratio.
Stability: Stable for a minimum of 18 months when stored at 2-8ºC in the original container and protected from light. The 1x diluted reagent is stable for several hours at room temperature when protected from light.
适用于: WBmore details
Store at +4°C. Please refer to protocols.
组件 | 1 kit |
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luminol reagent. | 1 x 20ml |
reaction buffer. | 2 x 20ml |
Abpromise™承诺保证使用ab5801于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | Use at an assay dependent concentration. Incubate membrane in chemiluminescent substrate for 1 minute then rinse off. |