Anti-Histone H3 (tri methyl K9，phospho S10)抗体
参阅全部 Histone H3 一抗
兔多克隆抗体to Histone H3 (tri methyl K9，phospho S10)
Rabbit
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
适用于: IHC-P, WB, ICC/IFmore details
与反应: Mouse, Cow, Human
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
WB: Calf Thymus Histone, HeLa Nuclear, NIH3T3 Nuclear. ICC/IF: SKN cells, HeLa cells. IHC-P: Human breast carcinoma.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
浓度
批次浓度范围 50 µg 浓度为 0.1 - 0.3 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab5819于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| WB | 1/500 - 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 17 kDa). | |
| ICC/IF | (1) | Use at an assay dependent concentration. |
Entrez Gene: 8350 Human
Entrez Gene: 8351 Human
Entrez Gene: 8352 Human
Entrez Gene: 8353 Human
Entrez Gene: 8354 Human
Entrez Gene: 8355 Human
Entrez Gene: 8356 Human
Entrez Gene: 8357 Human
Entrez Gene: 8358 Human
Entrez Gene: 8968 Human
Entrez Gene: 319152 Mouse
Entrez Gene: 319153 Mouse
Entrez Gene: 360198 Mouse
Entrez Gene: 97908 Mouse
Omim: 602810 Human
SwissProt: P68431 Human
SwissProt: P68433 Mouse
Unigene: 132854 Human
Unigene: 247813 Human
Unigene: 247814 Human
Unigene: 248176 Human
Unigene: 443021 Human
Unigene: 484990 Human
Unigene: 532144 Human
Unigene: 533292 Human
Unigene: 546315 Human
Unigene: 586261 Human
Unigene: 591778 Human
Unigene: 221301 Mouse
Unigene: 261657 Mouse
Unigene: 377874 Mouse
Unigene: 390558 Mouse
Unigene: 397328 Mouse
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Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K9, phospho S10) antibody (ab5819)
ab5819 staining Histone H3 (tri methyl K9, phospho S10) in mitotic HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab5819 at 0.5µg/ml and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 2µg/ml (shown in red). The secondary antibody (shown in green) was ab150081, Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h at room temperature. Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
Western blot - Anti-Histone H3 (tri methyl K9, phospho S10) antibody (ab5819)
All lanes : Anti-Histone H3 (tri methyl K9, phospho S10) antibody (ab5819) at 1 µg/ml
Lane 1 : Calf Thymus Histone at 0.5 µg
Lane 2 : HeLa Nuclear – Triton Prep at 10 µg
Lane 3 : NIH3T3 Nuclear – Triton Prep at 10 µg
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDa
Exposure time: 2 minutes
Blocking buffer: 2% BSA
Gel type: MES
Western blot - Anti-Histone H3 (tri methyl K9, phospho S10) antibody (ab5819)
All lanes : Anti-Histone H3 (tri methyl K9, phospho S10) antibody (ab5819) at 1/500 dilution
Lane 1 : HeLa histones
Lane 2 : Colcemid-treated HeLa histones
Lane 3 : HeLa histones with Human Histone H3 (tri methyl K9, phospho S10) peptide (ab15644) at 1 µg
Lane 4 : Colcemid-treated HeLa histones with Human Histone H3 (tri methyl K9, phospho S10) peptide (ab15644) at 1 µg
Lane 5 : HeLa histones with Human Histone H3 (unmodified) peptide (ab7228) at 1 µg
Lane 6 : Colcemid-treated HeLa histones with Human Histone H3 (unmodified) peptide (ab7228) at 1 µg
Lane 7 : HeLa histones with Human Histone H3 (phospho S10) peptide (ab11477) at 1 µg
Lane 8 : Colcemid-treated HeLa histones with Human Histone H3 (phospho S10) peptide (ab11477) at 1 µg
Lane 9 : HeLa histones with Human Histone H3 (tri methyl K9) peptide (ab1773) at 1 µg
Lane 10 : Colcemid-treated HeLa histones with Human Histone H3 (tri methyl K9) peptide (ab1773) at 1 µg
Lysates/proteins at 0.5 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 17 kDa
Observed band size: 22 kDawhy is the actual band size different from the predicted?
Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.
Rabbit polyclonal to Histone H3 (phospho S10, tri methyl K9) - ab5819, on histone preparations from HeLa cells. Primary antibody used at 1/500.
Lanes 1, 3, 5, 7, 9 - control HeLa histones (0.5ug/lane)
Lanes 2, 4, 6, 8, 10 - Colcemid-treated HeLa histones (0.5ug/lane)
All blocking peptides are 1ug/lane
Lanes 1 and 2: ab5819
Lanes 3 and 4: ab5819 + Histone H3 (Tri Me K9, phospho S10) peptide - ab15644
Lanes 5 and 6: ab5819 + Histone H3 peptide (unmodified) - ab7228
Lanes 7 and 8: ab5819 + Histone H3 (phospho S10) peptide - ab11477
Lanes 9 and 10: ab5819 + Histone H3 (tri methyl K9) peptide - ab1773
Secondary antibody - Goat polyclonal to rabbit IgG (HRP) - ab6721 1/5000.
ab5819 is specific for Histone H3 phosphorylated at residue S10 and tri methylated at residue K9. The activity of the antibody is blocked by the addition of the immunizing peptide, ab15644 (lanes 3 and 4).
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K9, phospho S10) antibody - ChIP Grade (ab5819)This image is courtesy of Darin McDonald, Hendzel Laboratory
SKN cells stained with ab5819 (green) at a dilution of 1/2000. The cells were fixed in paraformaldehyde for 10 minutes prior to incubation with ab5819. The DNA is stained with DAPI (blue). 100x magnification.
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K9, phospho S10) antibody - ChIP Grade (ab5819)
ab5819 at a 1/500 dilution staining mitotic human HeLa cells by immunocytochemistry. The antibody was incubated with formaldehyde fixed cells for 1 1/2 hours and bound antibody was then detected using a Texas red conjugated goat anti-rabbit IgG.
This image is courtesy of an Anonymous Abreview submitted on 6 April 2006.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (tri methyl K9, phospho S10) antibody - ChIP Grade (ab5819)
IHC image of Histone H3 (tri methyl K9, phospho S10) staining in human breast carcinoma FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab5819, 5µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
