Anti-EGFR (phospho Y1148)抗体
参阅全部 EGFR 一抗
兔多克隆抗体to EGFR (phospho Y1148)
Rabbit
适用于: WB, ICCmore details
与反应: Human
Synthetic peptide corresponding to Human EGFR (phospho Y1148).
A431 cells stimulated with EGF, A549 cells.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA
浓度
50 µl 浓度为 0.4 mg/ml
Immunogen affinity purified
The antibody has been negatively preadsorbed using (i) a non phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated epidermal growth factor receptor (EGFR), and (ii) a generic tyrosine phosphorylated peptide to remove antibody that is reactive with phosphotyrosine, irrespective of the sequence. The final product is generated by affinity chromatography using an EGFR-derived peptide that is phosphorylated at tyrosine 1148.
多克隆
IgG
Abpromise™承诺保证使用ab5651于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 140 kDa. | |
| ICC | 1/250. |
Entrez Gene: 1956 Human
Omim: 131550 Human
SwissProt: P00533 Human
Unigene: 488293 Human
Unigene: 605083 Human
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Western blot - Anti-EGFR (phospho Y1148) antibody (ab5651)
All lanes : Anti-EGFR (phospho Y1148) antibody (ab5651) at 1/1000 dilution
Lane 1 : A431 cells unstimulated with no peptide
Lane 2 : A431 cells stimulated with 200 ng/mL EGF for 15 minutes with no peptide
Lane 3 : A431 cells stimulated with 200 ng/mL EGF for 15 minutes with non-phosphopeptide corresponding to the phosphopeptide immunogen
Lane 4 : A431 cells stimulated with 200 ng/mL EGF for 15 minutes with a generic phosphotyrosine-containing peptide
Lane 5 : A431 cells stimulated with 200 ng/mL EGF for 15 minutes with phosphopeptide immunogen
Secondary
All lanes : goat F(ab’)2 anti-rabbit IgG HRP conjugate
Blocking buffer: 5% BSA-TBST
The phosphopeptide corresponding to EGFR [pY1148] blocks the signal.
Immunocytochemistry - Anti-EGFR (phospho Y1148) antibody (ab5651)
Immunocytochemical analysis of EGFR (pY1148) on A549 cells. The cells were fixed with 4% paraformaldehyde, permeabilized with 0.25% Triton™ X-100. The cells were labeled with ab5651 at 1/250 dilution in 1% BSA and incubated for 3 hours at room temperature followed by Alexa Fluor 488 Goat Anti-Rabbit IgG Secondary Antibody at a dilution of 1/400 (Panel a: green). Nuclei (Panel b: blue) were stained DAPI. (Panel c: red) F-actin was stained with Alexa Fluor 594 Phalloidin. Panel d is a merged image showing cytoplasmic localization. Panel e shows no primary antibody control.
