Anti-PHAPI2 / APRIL抗体
参阅全部 PHAPI2 / APRIL 一抗
山羊多克隆抗体to PHAPI2 / APRIL
Goat
This antibody does NOT recognize the TNF family member also known as APRIL - LocusLink Number 8741
适用于: IHC-P, WBmore details
与反应: Mouse, Rat, Human, Pig
预测可用于: Cow, Dog
Synthetic peptide: KRKRETDDEGEDD, corresponding to C terminal amino acids 237-249 of Human PHAPI2/ APRIL.
Human Tonsil lysate.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris, 0.5% BSA
浓度
100 µg 浓度为 0.5 mg/ml
Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
多克隆
IgG
Abpromise™承诺保证使用ab4224于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 29 kDa). 1 hour primary incubation is recommended for this product. |
Entrez Gene: 10541 Human
Entrez Gene: 67628 Mouse
SwissProt: Q92688 Human
SwissProt: Q9EST5 Mouse
Unigene: 494604 Human
Unigene: 263913 Mouse
Unigene: 20465 Rat
Acidic (leucine rich) nuclear phosphoprotein 32 family member B antibody
Acidic leucine rich nuclear phosphoprotein 32 family member B antibody
Acidic leucine-rich nuclear phosphoprotein 32 family member B antibody
Acidic nuclear phosphoprotein 32 family member B antibody
Acidic protein rich in leucines antibody
AN32B_HUMAN antibody
ANP 32B antibody
ANP32B antibody
APRIL antibody
OTTHUMP0000006377 antibody
PAL 31 antibody
PAL31 antibody
PHAPI 2 antibody
PHAPI2 antibody
PHAPI2 protein antibody
PHAPI2a antibody
Proliferation related acidic leucine rich protein antibody
Putative HLA-DR-associated protein I-2 antibody
Silver stainable protein SSP 29 antibody
Silver stainable protein SSP29 antibody
Silver-stainable protein SSP29 antibody
Ssp 29 antibody
Ssp29 antibody
Western blot - Anti-PHAPI2 / APRIL antibody (ab4224)
All lanes : Anti-PHAPI2 / APRIL antibody (ab4224) at 0.1 µg/ml
Lane 1 : Human tonsil lysate
Lane 2 : Rat spleen lysate
Lane 3 : Pig spleen lysate
Lysates/proteins at 35 mg/ml per lane.
Predicted band size: 29 kDa
Observed band size: 28-30 kDawhy is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot - Anti-PHAPI2 / APRIL antibody (ab4224)
Anti-PHAPI2 / APRIL antibody (ab4224) at 0.1 µg/ml + NIH3T3 cell lysate at 35 µg
Predicted band size: 29 kDa
Observed band size: 30 kDawhy is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot - Anti-PHAPI2 / APRIL antibody (ab4224)
ab4224 at a concentration of 1 µg/ml, staining ~30 kDa PHAP I2 in human lymph node lysate (RIPA bufer, 30 µg total proteion per lane) by western blot (primary incubated for 1 hour, detected by ECL). ab4224 at a concentration of 1 µg/ml, staining ~30 kDa PHAP I2 in human lymph node lysate (RIPA bufer, 30 µg total proteion per lane) by western blot (primary incubated for 1 hour, detected by ECL).
Western blot - Anti-PHAPI2 / APRIL antibody (ab4224)
Anti-PHAPI2 / APRIL antibody (ab4224) at 0.3 µg/ml + A431 lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 29 kDa
Observed band size: 29 kDa
