Anti-EIF2B5 (phospho S539) antibody

• 产品名称

  Anti-EIF2B5 (phospho S539)抗体
  参阅全部 EIF2B5 一抗

• 描述

  兔多克隆抗体to EIF2B5 (phospho S539)

• 宿主

  Rabbit

• 特异性

  This ab reacts with both human and rat (see, for example, PMID 21165642, 20484009 and 21832246) but it is not very clear if this recognition happens only when S535 (rat) and S544 (human) are phosphorylated or when S535 (rat)/S540 (human) are phosphorylated.

• 经测试应用

  适用于: WBmore details

• 种属反应性

  与反应: Mouse, Rat, Recombinant fragment
  

• 免疫原

  Synthetic peptide corresponding to Rat EIF2B5 (phospho S535). Phosphopeptide derived from the region of rat eIF2Be that contains serine 535 (corresponding to serine 539 in the human sequence).

• 阳性对照

• Recombinant eIF-2B epsilon treated with GSK 3 beta and lambda phosphatase, CHO-T cells transfected with human insulin receptor (IR) +/- insulin. L6, L6 differentiated to myotubes, L6 treated with Insulin (10nM for 10 min), C2C12 and C2C12 treated with Insulin (10nM for 10 min)

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

• 存储溶液

  pH: 7.30
  Preservative: 0.05% Sodium azide
  Constituents: PBS, 0.1% BSA
  
  BSA is IgG and protease free

• 浓度

• 50 µl 浓度为 0.5 mg/ml

• 纯度

  Immunogen affinity purified

• 纯化说明

  Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively pre-adsorbed using (i) a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated eukaryotic initiation factor 2B epsilon (eIF-2B epsilon), and (ii) a generic serine phosphorylated peptide to remove antibody that is reactive with phospho-serine, irrespective of the sequence. The final product is generated by affinity chromatography using an eIF-2B epsilon-derived peptide that is phosphorylated at serine 539.

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab4775于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 224045 Mouse

• Entrez Gene: 192234 Rat

• SwissProt: Q8CHW4 Mouse

• SwissProt: Q64350 Rat

• Unigene: 233855 Mouse

• Unigene: 10607 Rat

• 别名

• EIF 2B antibody

• eIF 2B GDP GTP exchange factor antibody

• eIF 2B GDP GTP exchange factor subunit epsilon antibody

• eIF-2B GDP-GTP exchange factor subunit epsilon antibody

• EIF2B antibody

• EIF2B5 antibody

• EIF2BE antibody

• EIF2Bepsilon antibody

• Eukaryotic Translation Initiation Factor 2 Beta Epsilon antibody

• Eukaryotic translation initiation factor 2B subunit 5 epsilon 82kDa antibody

• Eukaryotic translation initiation factor 2B subunit 5 epsilon antibody

• LVWM antibody

• Translation initiation factor eIF 2B subunit epsilon antibody

• Translation initiation factor eIF-2B subunit epsilon antibody

• CACH antibody

• CLE antibody

• EI2BE_HUMAN antibody

• 

  Western blot - Anti-EIF2B5 (phospho S539) antibody (ab4775)

  Peptide Competition: Recombinant eIF-2B episilon treated with lambda phosphatase (1) or treated with GSK-3 beta (lanes 2-5) and added to background extracts were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then were incubated with 0.50 µg/mL ab4775 antibody, following prior incubation with: no peptide (1, 2), the nonphosphopeptide corresponding to the immunogen (3), a generic phophoserine containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to ab4775 blocks the antibody signal, thereby demonstrating the specificity of the antibody. The data also show the up-regulation of the ab4775 phospho signal upon stimulation with GSK-3 beta. Pep

• 

  Western blot - Anti-EIF2B5 (phospho S539) antibody (ab4775)

  All lanes : Anti-EIF2B5 (phospho S539) antibody (ab4775) at 1/2000 dilution
  
  Lane 1 : L6 whole cell extract
  Lane 2 : L6 differentiated to myotubes
  Lane 3 : L6 treated with Insulin (10nM for 10 min)
  Lane 4 : C2C12
  Lane 5 : C2C12 treated with Insulin (10nM for 10 min)
  
  Lysates/proteins at 30 µg per lane.
  
  Secondary
  All lanes : Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/4000 dilution
  
  Predicted band size: 82 kDa
  
  
  
  

  A 80 kDa band corresponding to Phospho-eIF2b epsilon was observed across the cell lines tested and reduced upon treatment and in differentiated cell line.