Anti-MDA5 antibody

• 产品名称

  Anti-MDA5抗体
  参阅全部 MDA5 一抗

• 描述

  山羊多克隆抗体to MDA5

• 宿主

  Goat

• 经测试应用

  适用于: IHC-P, Flow Cytmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Synthetic peptide corresponding to Human MDA5 aa 2-14 (N terminal).
  Sequence:

  SNGYSTDENFRYL-C

  
  (Peptide available as ab23050)

  Run BLAST with Run BLAST with 

• 阳性对照

• IHC-P: Human pancreas tissue. Flow Cyt: K562 cells.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

• 存储溶液

  pH: 7.3
  Preservative: 0.02% Sodium azide
  Constituents: Tris buffered saline, 0.5% BSA

• 浓度

• 100 µg 浓度为 0.5 mg/ml

• 纯度

  Immunogen affinity purified

• 纯化说明

  Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab4544于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 64135 Human

• Omim: 606951 Human

• SwissProt: Q9BYX4 Human

• Unigene: 163173 Human

• 别名

• DEAD/H box polypeptide antibody

• Helicard antibody

• Helicase with 2 CARD domains antibody

• Hlcd antibody

• IDDM 19 antibody

• IDDM19 antibody

• IFIH 1 antibody

• Ifih1 antibody

• IFIH1_HUMAN antibody

• Interferon induced helicase C domain containing protein 1 antibody

• interferon induced with helicase C domain 1 antibody

• Interferon induced with helicase C domain protein 1 antibody

• Interferon-induced helicase C domain-containing protein 1 antibody

• Interferon-induced with helicase C domain protein 1 antibody

• MDA 5 antibody

• MDA-5 antibody

• Melanoma differentiation associated protein 5 antibody

• Melanoma differentiation-associated gene 5 antibody

• Melanoma differentiation-associated protein 5 antibody

• MGC133047 antibody

• Murabutide down regulated protein antibody

• Murabutide down-regulated protein antibody

• RH 116 antibody

• RH116 antibody

• RIG I like receptor 2 antibody

• RLR 2 antibody

• RNA helicase DEAD box protein 116 antibody

• RNA helicase-DEAD box protein 116 antibody

• CADM-140 autoantigen antibody

• Clinically amyopathic dermatomyositis autoantigen 140 kDa antibody

• DEAD/H (Asp Glu Ala Asp/His) box polypeptide antibody

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDA5 antibody (ab4544)

  ab4544 (10ug/ml) staining human MDA5 in human pancreas by immunohistochemistry using paraffin embedded tissue. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

  ab4544 shows strong cytoplasmic staining of some cells in the islets of Langerhans.

• 

  Flow Cytometry - Anti-MDA5 antibody (ab4544)

  Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 µg/ml) followed by Alexa Fluor 488® secondary antibody (1 µg/ml).

  IgG control: Non-immunized goat IgG (black line) followed by Alexa Fluor 488® secondary antibody.