Anti-FOXA2抗体
参阅全部 FOXA2 一抗
山羊多克隆抗体to FOXA2
Goat
This antibody is expected to recognise both reported isoforms.
适用于: WBmore details
与反应: Rat, Human
预测可用于: Chicken, Xenopus laevis
Synthetic peptide: GVYSRPIMNSS, corresponding to amino acids 447-457 of Human FOXA 2.
WB: Rat lung and human liver tissue lysates.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
浓度
100 µg 浓度为 0.5 mg/ml
Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
多克隆
IgG
Abpromise™承诺保证使用ab5074于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 48 kDa). A 1 hour primary incubation is recommended for this product. A nuclear extraction procedure may be required to concentrate the protein in samples. Read More |
Entrez Gene: 3170 Human
Omim: 600288 Human
SwissProt: Q9Y261 Human
Unigene: 155651 Human
Unigene: 10948 Rat
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Western blot - Anti-FOXA2 antibody (ab5074)
Anti-FOXA2 antibody (ab5074) at 1 µg/ml + Rat Lung tissue lysate at 35 µg
Predicted band size: 48 kDa
Observed band size: 60 kDawhy is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot - Anti-FOXA2 antibody (ab5074)
Anti-FOXA2 antibody (ab5074) at 1 µg/ml + Human Liver lysate at 1 µg/ml
Predicted band size: 48 kDa
Observed band size: 60 kDawhy is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.