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Anti-FOXA2 antibody

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  • 产品名称

    Anti-FOXA2抗体
    参阅全部 FOXA2 一抗

  • 描述

    山羊多克隆抗体to FOXA2

  • 宿主

    Goat

  • 特异性

    This antibody is expected to recognise both reported isoforms.

  • 经测试应用

    适用于: WBmore details

  • 种属反应性

    与反应: Rat, Human
    预测可用于: Chicken, Xenopus laevis

  • 免疫原

    Synthetic peptide: GVYSRPIMNSS, corresponding to amino acids 447-457 of Human FOXA 2.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 阳性对照

    • WB: Rat lung and human liver tissue lysates.

  • 常规说明


    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • 存储溶液

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 0.5% BSA

  • 浓度

    • 100 µg 浓度为 0.5 mg/ml

  • 纯度

    Immunogen affinity purified

  • 纯化说明

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

  • 克隆

    多克隆

  • 同种型

    IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab5074于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用Ab评论说明
WB

Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 48 kDa). 

A 1 hour primary incubation is recommended for this product. A nuclear extraction procedure may be required to concentrate the protein in samples.

Read More
  • 数据库链接

  • 别名

    • foxa2 antibody

    • FOXA2_HUMAN antibody

    • Hepatic nuclear factor 3 beta antibody

    • Hepatocyte nuclear factor 3 antibody

    • Hepatocyte nuclear factor 3 beta antibody

    • Hepatocyte nuclear factor 3-beta antibody

    • HNF 3B antibody

    • HNF-3-beta antibody

    • HNF-3B antibody

    • HNF3B antibody

    • MGC19807 antibody

    • TCF 3B antibody

    • TCF-3B antibody

    • TCF3B antibody

    • Transcription factor 3B antibody

    • Forkhead box A2 antibody

    • Forkhead box protein A2 antibody

    • FOX A2 antibody

  • Western blot - Anti-FOXA2 antibody (ab5074)

    Western blot - Anti-FOXA2 antibody (ab5074)

    Anti-FOXA2 antibody (ab5074) at 1 µg/ml + Rat Lung tissue lysate at 35 µg

    Predicted band size: 48 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour. Detected by chemiluminescence.

  • Western blot - Anti-FOXA2 antibody (ab5074)

    Western blot - Anti-FOXA2 antibody (ab5074)

    Anti-FOXA2 antibody (ab5074) at 1 µg/ml + Human Liver lysate at 1 µg/ml

    Predicted band size: 48 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour. Detected by chemiluminescence.