Anti-Rb (phospho T821) antibody

• 产品名称

  Anti-Rb (phospho T821)抗体
  参阅全部 Rb 一抗

• 描述

  兔多克隆抗体to Rb (phospho T821)

• 宿主

  Rabbit

• 经测试应用

  适用于: WBmore details

• 种属反应性

  与反应: Human
  预测可用于: Rat

• 免疫原

  Synthetic peptide corresponding to Human Rb (phospho T821).
  Database link: P06400

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

• 存储溶液

  pH: 7.30
  Preservative: 0.05% Sodium azide
  Constituents: PBS, 50% Glycerol, 0.1% BSA

• 纯度

  Immunogen affinity purified

• 纯化说明

  Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Rb protein. The final product is generated by affinity chromatography using a Rb-derived peptide that is phosphorylated at threonine 821.

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab4787于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 5925 Human

• Entrez Gene: 24708 Rat

• Omim: 614041 Human

• SwissProt: P06400 Human

• SwissProt: P33568 Rat

• Unigene: 408528 Human

• Unigene: 55115 Rat

• 别名

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• OSRC antibody

• 

  Western blot - Anti-Rb (phospho T821) antibody (ab4787)

  Peptide Competition: Cell extracts prepared from Jurkat cells in high growth phase were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. Membranes were incubated with 0.50 µg/mL ab4787, following prior incubation in the: absence of the phosphopeptide immunogen (1), a generic phosphothreonine containing peptide (2), the non phosphopeptide corresponding to the phosphopeptide immunogen (3), or the presence of the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar detection method. The data show that only the phosphopeptide corresponding to this site blocks the antibody signal, demonstrating the specificity of the ab4787 antibody for this epitope. Peptide Competition: Cell extracts prepared from Jurkat cells in high growth phase were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. Membranes were in