Anti-Rb (phospho S249)抗体
参阅全部 Rb 一抗
兔多克隆抗体to Rb (phospho S249)
Rabbit
适用于: WBmore details
与反应: Human
预测可用于: Mouse, Rat, Chicken
Synthetic peptide corresponding to Human Rb (phospho S249 + T252).
Database link: P06400
Jurkat cells in high growth phase.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
浓度
50 µl 浓度为 0.2 mg/ml
Immunogen affinity purified
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Rb protein. The final product is generated by affinity chromatography using a Rb-derived peptide that is phosphorylated at serine 249 and threonine 252.
多克隆
IgG
Abpromise™承诺保证使用ab4788于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
应用说明
WB: 1/1000. Detects a band of approximately 120 kDa (predicted molecular weight: 106 kDa).
Can be blocked with Rb peptide - phospho S249 + T252 (phospho and non-phospho pair).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Entrez Gene: 386582 Chicken
Entrez Gene: 5925 Human
Entrez Gene: 19645 Mouse
Omim: 614041 Human
SwissProt: Q90600 Chicken
SwissProt: P06400 Human
SwissProt: P13405 Mouse
Unigene: 408528 Human
Unigene: 273862 Mouse
Unigene: 55115 Rat
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Western blot - Anti-Rb (phospho S249) antibody (ab4788)
Peptide Competition: Cell extracts prepared from Jurkat cells in high growth phase were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. Membranes were incubated with 0.50 µg/mL ab4788, following prior incubation in the absence of the phosphopeptide immunogen (1), a generic phosphoserine containing peptide (2), a generic phosphothreonine containing peptide (3), the non-phosphopeptide corresponding to the phosphopeptide immunogen (4), or the presence of the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar detection method. The data show that only the phosphopeptide corresponding to this site blocks the antibody signal, therefore demonstrating the specificity of the ab4788 antibody for this epitope. Peptide Competition: Cell extracts prepared from Jurkat cells in high growth phase were resolved by SDS-PAGE on a 1
