Anti-MCM3抗体
参阅全部 MCM3 一抗
兔多克隆抗体to MCM3
Rabbit
适用于: ICC/IF, IP, WB, IHC-Pmore details
与反应: Mouse, Human
预测可用于: Orangutan
Synthetic peptide within Human MCM3 aa 500-600. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_002379.2 (GeneID 4172).
Database link: P25205
WB: HeLa cell RIPA extract. HeLa OHIO extract. IHC-P: Human colon carcinoma tissue. ICC/IF: HeLa cells.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab4460于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | Use a concentration of 1 µg/ml. | |
| IP | Use at 2-10 µg/mg of lysate. | |
| WB | (2) | 1/2000 - 1/10000. Detects a band of approximately 105 kDa (predicted molecular weight: 90 kDa). |
| IHC-P | 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Entrez Gene: 4172 Human
Entrez Gene: 17215 Mouse
Omim: 602693 Human
SwissProt: P25205 Human
SwissProt: P25206 Mouse
Unigene: 179565 Human
Unigene: 4502 Mouse
DNA polymerase alpha holoenzyme-associated protein P1 antibody
DNA replication factor MCM3 antibody
DNA replication licensing factor mcm3 antibody
HCC 5 antibody
HCC5 antibody
hRlf beta subunit antibody
Human cervical cancer proto oncogene 5 antibody
MCM 3 antibody
mcm3 antibody
MCM3 minichromosome maintenance deficient 3 antibody
MCM3_HUMAN antibody
MGC1157 antibody
Minichromosome maintenance complex component 3 antibody
Minichromosome maintenance deficient 3 antibody
Minichromosome maintenance protein 3 antibody
P1 h antibody
P1 MCM3 antibody
P1 Protein antibody
P1-MCM3 antibody
P1.h antibody
p102 antibody
P102 protein antibody
Replication licensing factor beta subunit antibody
RLF beta subunit antibody
RLF subunit beta antibody
RLFB antibody
Cervical cancer proto oncogene 5 antibody
DNA polymerase alpha holoenzyme associated P1 antibody
DNA polymerase alpha holoenzyme associated protein P1 antibody
Western blot - Anti-MCM3 antibody (ab4460)
Whole cell lysate (10 µg) from HeLa, Jurkat, HEK-293T, and TCMK-1 cells prepared using NETN lysis buffer.
ab4460 used for WB at 0.04 µg/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 antibody (ab4460)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human small cell lung cancer tissue labelling MCM3 with ab4460 at 1/1000 (1µg/ml). Detection: DAB.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 antibody (ab4460)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling MCM3 with ab4460 at 1/1000 (1µg/ml). Detection: DAB.
Immunoprecipitation - Anti-MCM3 antibody (ab4460)
Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer.
Lanes 1 and 2: ab4460 used for IP at 6 µg per reaction, two lots tested.
Lane 3 Control IgG.
For blotting immunoprecipitated MCM3, ab4460 was used at 0.04 µg/ml.
Western blot - Anti-MCM3 antibody (ab4460)
HeLa cell RIPA extract (50 µg) incubated with ab4460 used at 0.2 µg/ml for 1 hour. HeLa cell RIPA extract (50 µg) incubated with ab4460 used at 0.2 µg/ml for 1 hour.
Western blot - Anti-MCM3 antibody (ab4460)
WB using ab4460. Western blot analysis of ab4460 on HeLa OHIO extract.
Immunocytochemistry/ Immunofluorescence - Anti-MCM3 antibody (ab4460)
ICC/IF image of ab4460 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4460, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
