Anti-Peripherin抗体
参阅全部 Peripherin 一抗
兔多克隆抗体to Peripherin
Rabbit
适用于: ICC/IF, WBmore details
与反应: Rat, Human
Recombinant full length rat peripherin purified from E. coli.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Preservative: 0.01% Sodium azide
Whole antiserum
多克隆
IgG
Abpromise™承诺保证使用ab4666于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (3) | Use at an assay dependent concentration. |
| WB | 1/1000 - 1/2000. Detects a band of approximately 57 kDa. |
Entrez Gene: 5630 Human
Omim: 170710 Human
SwissProt: P41219 Human
Unigene: 37044 Human
Unigene: 11086 Rat
Neurofilament 4 antibody
Perf antibody
PERI_HUMAN antibody
Peripherin antibody
PRPH 1 antibody
prph antibody
PRPH1 antibody
NEF 4 antibody
NEF4 antibody
Neurofilament 4 (57kD) antibody
Immunocytochemistry/ Immunofluorescence - Anti-Peripherin antibody (ab4666)
Immunocytochemistry/Immunofluorescence analysis of mixed neuron/glia cultures from newborn rat brain labelling Peripherin (green) with ab4666 and Neurofilament heavy polypeptide (red) with ab4680. A class of large neurons, like the one at the top right of this image, contain peripherin, while the majority of neurons and their processes contain Neurofilament heavy polypeptide and not peripherin. The blue channel reveals the localization of DNA.
Western blot - Anti-Peripherin antibody (ab4666)
Anti-Peripherin antibody (ab4666) at 1/1000 dilution + Rat spinal cord homogenate
Observed band size: 57 kDawhy is the actual band size different from the predicted?
Western blot - Anti-Peripherin antibody (ab4666)
Anti-Peripherin antibody (ab4666) at 1 µg/ml + Recombinant Human Peripherin protein (ab114335) at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 20 seconds
Secondary antibody - goat anti-rabbit HRP preasorbed (ab97080)
