Anti-ATG8抗体
兔多克隆抗体to ATG8
Rabbit
适用于: WB, ELISAmore details
与反应: Saccharomyces cerevisiae
Recombinant full length protein (S. cerevisiae).
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride
浓度
250 µg 浓度为 5 mg/ml
250 µg 浓度为 5 mg/ml
Ion Exchange Chromatography
Anti-Apg8 antibody, also known as Atg8, is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.
多克隆
IgG
Abpromise™承诺保证使用ab4753于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (4) | 1/4000 - 1/8000. Detects a band of approximately 14 kDa. This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or their conjugates). Other intrinsic bands are readily detectable in yeast lysates at lower antibody dilutions. |
| ELISA | 1/20000 - 1/100000. |
Entrez Gene: 852200 Saccharomyces cerevisiae
SwissProt: P38182 Saccharomyces cerevisiae
AUT7 antibody
CVT5 antibody
Apg 8 antibody
APG8 antibody
Atg8p antibody
Western blot - Anti-ATG8 antibody (ab4753)
Ab4753, generated by immunization with recombinant yeast ATG8 (or Apg 8), was tested by immunoblot with other anti-UBL (Ubiquitin-like modifier) antibodies against E.coli lysates expressing the ATG8-GFP fusion protein (Apg 8-GFP in both panels)). All UBLs possess limited homology to Ubiquitin and to each other, therefore it is important to know the degree of reactivity of each antibody against each UBL.
Panel A shows total protein staining using ponceau.
Panel B shows specific reaction with ATG8 (Apg 8) using a 1:4,000 and 1:8,000 dilution of ab4753 followed by reaction with a 1:15,000 dilution of HRP Goat-a-Rabbit IgG (ab7090). All primary antibodies were diluted in TTBS buffer supplemented with 5% non-fat milk and incubated with the membranes overnight at 4° C. E.coli lysate proteins were separated by SDS-PAGE using a 15% gel.
Similar experiments (data not shown), where other UBL fusion proteins were separated and probed with this antibody showed no react
Western blot - Anti-ATG8 antibody (ab4753)This image is courtesy of an anonymous abreview.
Anti-ATG8 antibody (ab4753) at 1/1500 dilution + Whole cell lysate prepared from Human huh-7 cells expressing the Saccharomyces cerevisiae protein at 15 µg
Secondary
Sheep anti-rabbit IgG conjugated to HRP at 1/7000 dilution
Observed band size: 15 kDawhy is the actual band size different from the predicted?
Exposure time: 4 minutes
Gel run under denaturing conditions.
Primary antibody incubated for 10 minutes at 20°C.
Blocked using 0.5% milk for 1 minute at 20°C.
Detection method: Western lightning chemiluminescent reagent.
