Anti-Fibrillarin antibody [38F3] - Nucleolar Marker

• 产品名称

  Anti-Fibrillarin抗体[38F3] - Nucleolar Marker
  参阅全部 Fibrillarin 一抗

• 描述

  小鼠单克隆抗体[38F3] to Fibrillarin - Nucleolar Marker

• 宿主

  Mouse

• 特异性

  This clone was selected because it stains a single ~34kDa band on western blotting and shows a clear and strong punctate staining of yeast nuclei. It can therefore be used to identify nucleoli immunocytochemically. ab4566 was raised against yeast nuclear preps and the immunogen was identified as Nop1p, the yeast homolog of fibrillarin. Due to high aa homology the antibody should work with any specie possessing a nucleus, however this has not been tested.

• 经测试应用

  适用于: Flow Cyt, ICC/IF, WBmore details

• 种属反应性

  与反应: Mouse, Rat, Human
  预测可用于: Plants

• 免疫原

  Tissue, cells or virus corresponding to Saccharomyces cerevisiae Fibrillarin.

  Yeast nuclear preparation (S. cerevisiae). Hybridomas were screened by immunofluorescence on yeast cells and by western blotting on yeast protein homogenates (S. cerevisiae).

• 阳性对照

• ICC: HEK-293 cells and SH-SY5Y cells; WB: HEK-293, C6, NIH-3T3 nuclear fractions

• 常规说明

  Pfam number: PF01269. A reference below describes the characterization of D77, an antibody very similar but not identical to ab4566.

  Gives a much weaker signal in western blot compared to ab218846.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

• 存储溶液

  Preservative: 0.065% Sodium azide
  Constituent: Tissue culture supernatant

• 纯度

  Tissue culture supernatant

• 纯化说明

  Sterile filtered.

• 克隆

  单克隆

• 克隆编号

  38F3

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab4566于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 2091 Human

• Entrez Gene: 102643269 Mouse

• Entrez Gene: 14113 Mouse

• Entrez Gene: 292747 Rat

• Omim: 134795 Human

• SwissProt: P22087 Human

• SwissProt: P35550 Mouse

• SwissProt: P22509 Rat

• Unigene: 299002 Human

• Unigene: 4595 Mouse

• Unigene: 98803 Rat

别名

• 34 kD nucleolar scleroderma antigen antibody

• 34 kDa nucleolar scleroderma antigen antibody

• Fbl antibody

• FBRL_HUMAN antibody

• FIB antibody

• FIB1 antibody

• FLRN antibody

• Histone-glutamine methyltransferase antibody

• Nop1p antibody

• RNA U3 small nucleolar interacting protein 1 antibody

• RNU3IP1 antibody

• rRNA 2' O methyltransferase fibrillarin antibody

• rRNA 2'-O-methyltransferase fibrillarin antibody

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody [38F3] - Nucleolar Marker (ab4566)

  Rat neurons and glial stained with mouse monoclonal to Fibrillarin (green) and with chicken antibody to neurofilament NF-H (red). Cells were counterstained with a fluorescent DNA probe (blue). Nuclear DNA is revealed with Hoechst dye (blue). Cultures were processed using our standard fixation and staining procedure (in protocol section).

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody [38F3] - Nucleolar Marker (ab4566)

  ICC analysis of HeLa cells stained with mouse monoclonal to Fibrillarin (green) and with chicken antibody to vimentin (red) and counterstained with a fluorescent DNA probe (blue). Nuclear DNA is revealed with DAPI(blue). The vimentin antibody was used at a dilution of 1/1000 and the fibrillarin monoclonal at 1/100. Cultures were processed using standard fixation and staining procedure (in protocol section).

• 

  Western blot - Anti-Fibrillarin antibody [38F3] - Nucleolar Marker (ab4566)

  All lanes : Anti-Fibrillarin antibody [38F3] - Nucleolar Marker (ab4566) at 1/500 dilution
  
  Lane 2 : C6 cytosol fraction
  Lane 3 : C6 nuclear fraction
  Lane 4 : HEK-293 cytosol fraction
  Lane 5 : HEK-293 nuclear fraction
  Lane 6 : NIH-3T3 cytosol fraction
  Lane 7 : NIH-3T3 nuclear fraction
  
  Observed band size: 37 kDawhy is the actual band size different from the predicted?
  
  

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody [38F3] - Nucleolar Marker (ab4566)

  Human neuroblastoma line SH-SY5Y stained with mouse monoclonal to Fibrillarin (green) and with chicken antibody to neurofilament NF-H (red) and counterstained with a fluorescent DNA probe (blue). Nuclear DNA is revealed with Hoechst dye (blue). The NF-H antibody was used at a dilution of 1/100000 and the fibrillarin monoclonal at 1/1000. Cultures were processed using standard fixation and staining procedure (in protocol section).

• 

  Western blot - Anti-Fibrillarin antibody [38F3] - Nucleolar Marker (ab4566)Image courtesy of an anonymous Abreview.

  Mouse embryonic fibroblast fractionation.
  Cytopl - cytoplasmic fraction.
  Nucl - nuclear fraction.
  20 µg of each loaded.
  
  ab4566 used at a 1/2000 dilution.
  The secondary used was an Alexa-Fluor 680 conjugated goat anti-mouse polyclonal used at a 1/10000 dilution.

  See Abreview

• 

  Western blot - Anti-Fibrillarin antibody [38F3] - Nucleolar Marker (ab4566)Image is courtesy of Dr Svetlana Khoronenkova

  All lanes : Anti-Fibrillarin antibody [38F3] - Nucleolar Marker (ab4566) at 1/2000 dilution (incubated for 1 hour, diluted with Blocking buffer 1:1 in PBS+0.1%Tween)
  
  Lane 1 : cytoplasmic protein fraction of HeLa cells with LI-COR® Odyssey® Blocking Buffer, 45 minutes at room temperature at 50 %
  Lane 2 : nuclear protein fraction of HeLa cells with LI-COR® Odyssey® Blocking Buffer, 45 minutes at room temperature at 50 %
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  All lanes : AlexaFluor 680 goat anti-mouse at 1/10000 dilution
  
  Performed under reducing conditions.
  
  Additional bands at: 34 kDa. We are unsure as to the identity of these extra bands.
  
  
  Exposure time: 1 second
  

  See Abreview

• 

  Flow Cytometry - Anti-Fibrillarin antibody [38F3] - Nucleolar Marker (ab4566)

  Overlay histogram showing HEK293 cells stained with ab4566 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab4566, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
  
  

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody [38F3] - Nucleolar Marker (ab4566)Image is courtesy of Cesar Camacho

  ab staining Fibrillarin in Human melanoma A7 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton and blocked with 1% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/500 in 1% BSA) for 24 hours at 4°C. A FITC-conjugated Goat anti-mouse polyclonal (1/200) was used as the secondary antibody.

  See Abreview

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody [38F3] - Nucleolar Marker (ab4566)

  High magnification view of human Hek293 cell nuclei stained with mouse monoclonal to fibrillarin (green), counterstained with a fluorescent DNA probe (blue). Nuclear DNA is revealed with Hoechst dye (blue). Cultures were processed using our standard fixation and staining procedure (in protocol section).