Anti-Neurofilament heavy polypeptide antibody

• 产品名称

  Anti-Neurofilament heavy polypeptide抗体
  参阅全部 Neurofilament heavy polypeptide 一抗

• 描述

  鸡多克隆抗体to Neurofilament heavy polypeptide

• 宿主

  Chicken

• 经测试应用

  适用于: ICC, IHC-FrFl, WBmore details

• 种属反应性

  与反应: Mouse, Rat, Cow, Human
  

• 免疫原

  Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C. Do Not Freeze.

• 存储溶液

  Preservative: 0.032% Sodium azide
  Constituent: PBS

• 纯度

  IgY fraction

• 纯化说明

  This is an IgY prep, similar to a mammalian ammonium sulphate IgG prep from serum. It is virtually pure IgY and we estimate that about 5% of material is specific to the immunogen.

• 克隆

  多克隆

• 同种型

  IgY

The Abpromise guarantee

Abpromise™承诺保证使用ab4680于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 528842 Cow

• Entrez Gene: 4744 Human

• Entrez Gene: 380684 Mouse

• Entrez Gene: 24587 Rat

• Omim: 162230 Human

• SwissProt: P12036 Human

• SwissProt: P19246 Mouse

• SwissProt: P16884 Rat

• Unigene: 198760 Human

• Unigene: 298283 Mouse

• Unigene: 108194 Rat

别名

• 200 kDa neurofilament protein antibody

• CMT2CC antibody

• Nefh antibody

• Neurofilament heavy polypeptide 200kDa antibody

• Neurofilament heavy polypeptide antibody

• Neurofilament triplet H protein antibody

• NF H antibody

• NF-H antibody

• NFH antibody

• NFH_HUMAN antibody

• 

  Immunohistochemistry - Free Floating - Anti-Neurofilament heavy polypeptide antibody (ab4680)

  Immunohistological analysis of a rat cerebellum section stained with ab4680 at a dilution 1:5,000 in red, and co-stained with rabbit pAb to GFAP at a dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA.

  Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 μM, and free floating sections were stained.

• 

  Western blot - Anti-Neurofilament heavy polypeptide antibody (ab4680)

  All lanes : Anti-Neurofilament heavy polypeptide antibody (ab4680) at 1/20000 dilution
  
  Lane 1 : Rat spinal cord lysate
  Lane 2 : Mouse spinal cord lysate
  Lane 3 : Cow spinal cord lysate
  
  Predicted band size: 200-220 kDa
  
  

• 

  Immunocytochemistry - Anti-Neurofilament heavy polypeptide antibody (ab4680)

  Mixed neuron/glial cultures stained with a mouse monoclonal antibody to neurofilament subunit NF-L (green) and ab4680, chicken antibody to neurofilament heavy polypeptide. This antibody binds primarily to the phosphorylated axonal forms of NF-H, in contrast to the NF-L antibody which stains both axonal and dendritic/perikaryal neurofilaments. The NF-L antibody therefore reveals a prominent cell body in green, while the surrounding axonal profiles are orange, since the are bound by both NF-L and the chicken NF-H antibody. Blue is a DNA stain.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Neurofilament heavy polypeptide antibody (ab4680)

  Human neuroblastoma SH-SY5Y cells stained with chicken anti-NF-H (red) and mouse monoclonal to fibrillarin 38F3 (green). Nuclear DNA is revealed with Hoechst dye (blue). The NF-H antibody was used at a dilution of 1/100000 and the fibrillarin monoclonal at 1/1000. Cultures were processed using our standard fixation and staining procedure (in protocol section).

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Neurofilament heavy polypeptide antibody (ab4680)

  Rat Neurons stained with chicken anti-NF-H (green). The NF-H antibody was used at a dilution of 1/100000 using our standard fixation and staining procedure (in protocol section). 

• 

  Immunocytochemistry - Anti-Neurofilament heavy polypeptide antibody (ab4680)Image from Wirt SE et al, J Cell Biol. 2010 Nov 15;191(4):809-25. Epub 2010 Nov 8, Fig 4. DOI 10.1083/jcb.201003048

  ab4680 staining Neurofilament heavy polypeptide (green) in murine embryoid bodies by Immunocytochemistry/ Immunofluorescence. Nuclei were visualized with DAPI.