Anti-Chk2 (phospho T68)抗体
参阅全部 Chk2 一抗
兔多克隆抗体to Chk2 (phospho T68)
Rabbit
ab3501 was tested using MCF7 cells treated with different amounts of doxorubicin (a DNA damaging agent). As the treatment increased the WB signal at 60kDa representing Chk2 increased, indicating that ab3501 was detecting the phosphorylated form of the protein. It has also been shown by peptide ELISA to be specific for the phosphorylated form of the immunizing peptide versus the non-phosphorylated form.
Reactivity occurs against human CHK2 pT68 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human CHK2 is minimal by ELISA. The antibody does not cross-react with Chk2 phosphorylated at other sites. A BLAST analysis was used to suggest reactivity with this protein from human and chimpanzee based on 100% homology for the immunogen sequence. Cross reactivity with CHK2 protein from mouse and rat may occur as sequence homology varies by one amino acid residues in this sequence (90% homology). Cross reactivity with CHK2 homologues from other sources has not been determined.
适用于: WBmore details
与反应: Human
预测可用于: Mouse, Rat, Chimpanzee
Synthetic peptide corresponding to Chk2 aa 50-150 (phospho T68).
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Immunogen affinity purified
This affinity purified antibody is directed against the phosphorylated form of human CHK2 at the pT68 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide.
多克隆
IgG
Abpromise™承诺保证使用ab3501于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) | 1/200 - 1/2000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa). |
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Unigene: 279308 Mouse
Entrez Gene: 11200 Human
Entrez Gene: 50883 Mouse
Omim: 604373 Human
SwissProt: O96017 Human
SwissProt: Q9Z265 Mouse
Unigene: 291363 Human
Unigene: 505297 Human
CDS 1 antibody
Cds1 antibody
Cds1 homolog antibody
Checkpoint kinase 2 antibody
Checkpoint like protein CHK2 antibody
CHEK 2 antibody
Chek2 antibody
Chk 2 antibody
CHK2 checkpoint homolog (S. pombe) antibody
CHK2 checkpoint homolog antibody
CHK2_HUMAN antibody
hCds1 antibody
HuCds 1 antibody
LFS 2 antibody
LFS2 antibody
PP1425 antibody
RAD 53 antibody
RAD53 antibody
Rad53 homolog antibody
Serine/threonine protein kinase Chk2 antibody
Serine/threonine-protein kinase Chk2 antibody
Western blot - Anti-Chk2 (phospho T68) antibody (ab3501)
The Western blot shows lysates from MCF-7 cells treated for 24 hours with increasing concentrations of the DNA damaging agent Doxorubicin (0-10µM). The blot clearly shows that Chk2 phosphorylation is increased with the higher concentrations of Doxorubicin.
