Anti-HLTF抗体
参阅全部 HLTF 一抗
兔多克隆抗体to HLTF
Rabbit
适用于: WBmore details
与反应: Mouse, Human
预测可用于: Rat, Rabbit, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
Synthetic peptide corresponding to HLTF aa 600-700.
Database link: 6596
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
Immunogen affinity purified
Antibody was affinity purified using an epitope specific to SMARCA3 immobilized on solid support.
多克隆
IgG
Abpromise™承诺保证使用ab17984于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | 1/3000 - 1/10000. Detects a band of approximately 125 kDa (predicted molecular weight: 114 kDa). |
Entrez Gene: 6596 Human
Entrez Gene: 20585 Mouse
Omim: 603257 Human
SwissProt: Q14527 Human
SwissProt: Q6PCN7 Mouse
Unigene: 3068 Human
Unigene: 209650 Mouse
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Western blot - Anti-HLTF antibody (ab17984)
All lanes : Anti-HLTF antibody (ab17984) at 0.5 µg/ml
Lane 1 : NIH 3T3 whole cell lysate
Lane 2 : TCMK-1 whole cell lysate
Lane 3 : 4T1 whole cell lysate
Lane 4 : CT26.WT whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 114 kDa
Western blot - Anti-HLTF antibody (ab17984)
Detection of SMARCA3 by western blot and immunoprecipitation.
Lane 1: 20 µg of HeLa nuclear extract
Lane 2: 50 µg of HeLa nuclear extract
LAne 3: 1 mg of HeLa nuclear extract immunoprecipitated by anti-SMARCA3 (ab17984), used at 4 µg/mg of lysate.
Anti-SMARCA3 (ab17984) was used for western blot analysis at 1/5,000.
Detection: chemiluminescence with an exposure time of 4 minutes.
Detection of SMARCA3 by western blot and immunoprecipitation.
Lane 1: 20 µg of HeLa nuclear extract
Lane 2: 50 µg of HeLa nuclear extract
LAne 3: 1 mg of HeLa nuclear extract immunoprecipitated by anti-SMARCA3 (ab17984), used at 4 µg/mg of lysate.
Anti-SMARCA3 (ab179