Anti-SV40 T-antigen抗体[PAb416]
参阅全部 SV40 T-antigen 一抗
小鼠单克隆抗体[PAb416] to SV40 T-antigen
Mouse
适用于: ICC/IF, WBmore details
与反应: Species independent
SV40 transformed mouse cells
Within amino acids 83-128 of the large T-antigen.
SV80 or SV-T2 cells.
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Liquid
Shipped at 4°C. Store at +4°C.
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS
Tissue culture supernatant
单克隆
PAb416
NS1
IgG2a
Abpromise™承诺保证使用ab16879于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (2) | Use a concentration of 1 - 5 µg/ml. |
| WB | (4) | Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 82 kDa. |
Nuclear
Large T antigen antibody
LT AG antibody
LT antibody
LTAG antibody
![Western blot - Anti-SV40 T-antigen antibody [PAb416] (ab16879)](https://www.abcam.cn/ps/products/16/ab16879/Images/ab16879-25763-anti-sv40-t-antigen-antibody-pab416-western-blot.jpg)
Western blot - Anti-SV40 T-antigen antibody [PAb416] (ab16879)This image is courtesy of an Abreview submitted by Mr Adam Hoffhines
All lanes : Anti-SV40 T-antigen antibody [PAb416] (ab16879) at 1 µg/ml
Lane 1 : Whole cell lysate mouse embryonic fibroblasts immortalized by transduction with a virus harboring the gene encoding the large T antigen.
Lane 2 : Whole cell lysate mouse embryonic fibroblasts, not immortalized.
Lysates/proteins at 7 µg per lane.
Secondary
All lanes : HRP conjugated sheep-anti mouse IgG
Developed using the ECL technique.
Performed under non-reducing conditions.
Predicted band size: 82 kDa
Observed band size: ~80 kDawhy is the actual band size different from the predicted?
Exposure time: 2 seconds
![Immunocytochemistry/ Immunofluorescence - Anti-SV40 T-antigen antibody [PAb416] (ab16879)](https://www.abcam.cn/ps/products/16/ab16879/Images/ab16879-25764-anti-sv40-t-antigen-antibody-pab416-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SV40 T-antigen antibody [PAb416] (ab16879)This image is courtesy of an anonymous Abreview
ab16879 at a 1/50 dilution staining SV40 transfected mouse keratinocytes by ICC/IF.
