Anti-n-Myc/MYCN antibody [NCM II 100]

• 产品名称

  Anti-n-Myc/MYCN抗体[NCM II 100]
  参阅全部 n-Myc/MYCN 一抗

• 描述

  小鼠单克隆抗体[NCM II 100] to n-Myc/MYCN

• 宿主

  Mouse

• 特异性

  This antibody reacts with N-myc/MYCN encoded proteins and their cleavage products.

• 经测试应用

  适用于: IP, IHC-Fr, ICC/IF, WB, ChIP, Flow Cytmore details

• 种属反应性

  与反应: Mouse, Human
  

• 免疫原

  Fusion protein corresponding to Human n-Myc/MYCN. Recombinant fusion protein. Original clone reference: PubMed ID - 2426708.
  Database link: P04198

• 阳性对照

• IMR5 Cells

• 常规说明

  This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

• 存储溶液

  Constituents: 0.82% Sodium phosphate, 50% Glycerol

• 纯度

  Tissue culture supernatant

• 纯化说明

  Purified from TCS.

• 克隆

  单克隆

• 克隆编号

  NCM II 100

• 骨髓瘤

  Sp2/0

• 同种型

  IgG1

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab16898于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 4613 Human

• Entrez Gene: 18109 Mouse

• Omim: 164840 Human

• SwissProt: P04198 Human

• SwissProt: P03966 Mouse

• Unigene: 25960 Human

• Unigene: 16469 Mouse

• 别名

• mycn antibody

• MYCN_HUMAN antibody

• N myc antibody

• N myc proto oncogene protein antibody

• N-myc proto-oncogene protein antibody

• Neuroblastoma derived v myc avian myelocytomatosis viral related oncogene antibody

• Neuroblastoma MYC oncogene antibody

• NMYC antibody

• NMYC proto oncogene protein antibody

• ODED antibody

• Oncogene NMYC antibody

• pp65/67 antibody

• V myc avian myelocytomatosis viral oncogene neuroblastoma derived homolog antibody

• v myc avian myelocytomatosis viral related oncogene neuroblastoma derived antibody

• v myc myelocytomatosis viral related oncogene neuroblastoma derived antibody

• bHLHe37 antibody

• Class E basic helix-loop-helix protein 37 antibody

• MODED antibody

• 

  Immunoprecipitation - Anti-n-Myc/MYCN antibody [NCM II 100] (ab16898)

  Immunoprecipitation of N-Myc/MYCN from S35 methionine-labeled IMR-5 cells using ab16898 (lane 1) and Normal Mouse IgG (lane 2).
  ab16898 used at 1 mg/sample.

  This image was generated using the ascites version of the product.

• 

  Flow Cytometry - Anti-n-Myc/MYCN antibody [NCM II 100] (ab16898)

  Overlay histogram showing SH-SY5Y cells stained with ab16898 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16898, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

  This image was generated using the ascites version of the product.