Anti-BRCA1 antibody [MS13]

• 产品名称

  Anti-BRCA1抗体[MS13]
  参阅全部 BRCA1 一抗

• 描述

  小鼠单克隆抗体[MS13] to BRCA1

• 宿主

  Mouse

• 经测试应用

  适用于: Flow Cyt (Intra), ICC/IF, IHC-Pmore details

• 种属反应性

  与反应: Human
  预测可用于: African green monkey

• 免疫原

  Recombinant full length protein corresponding to Human BRCA1.

• 表位

  Within the N-terminal 304 amino acids of BRCA1.

• 阳性对照

• IHC-P: Normal breast tissue. ICC/IF: MCF7 cells. Flow Cyt (Intra): MCF7 cells.

• 常规说明

  
  

  This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.02% Sodium azide
  Constituents: PBS, 6.97% L-Arginine

• 浓度

• 批次浓度范围 10 µg 浓度为 0.8 - 1 mg/ml

• 100 µg 浓度为 1 mg/ml

• 纯度

  Affinity purified

• 克隆

  单克隆

• 克隆编号

  MS13

• 骨髓瘤

  NS1

• 同种型

  IgG1

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab16781于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 672 Human

• Omim: 113705 Human

• SwissProt: P38398 Human

• Unigene: 194143 Human

• 别名

• BRCA1/BRCA2 containing complex subunit 1 antibody

• BRCA1/BRCA2-containing complex, subunit 1 antibody

• BRCA1_HUMAN antibody

• BRCAI antibody

• BRCC 1 antibody

• BRCC1 antibody

• Breast and ovarian cancer susceptibility protein 1 antibody

• Breast Cancer 1 antibody

• Breast Cancer 1 Early Onset antibody

• Breast cancer type 1 susceptibility protein antibody

• BROVCA1 antibody

• FANCS antibody

• IRIS antibody

• PNCA4 antibody

• PPP1R53 antibody

• Protein phosphatase 1 regulatory subunit 53 antibody

• PSCP antibody

• RING finger protein 53 antibody

• RNF53 antibody

• BRCA 1 antibody

• BRCA1 antibody

• BRCA1 DNA repair associated antibody

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCA1 antibody [MS13] (ab16781)

  IHC image of BRCA1 staining in a section of formalin-fixed paraffin-embedded normal human breast* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab16781, 5ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

• 

  Immunocytochemistry/ Immunofluorescence - Anti-BRCA1 antibody [MS13] (ab16781)

  ab16781 staining BRCA1 in MCF7 cells. The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab16781 at 1μg/ml concentration and ab6046 at 1/1000 dilution overnight at +4°C, followed by a further incubation at room temperature for 1h with Goat Anti-Mouse IgG H&L (ab150117) at 1/1000 dilution (shown in green) and Goat anti-Rabbit IgG (ab150080) at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labelled in blue with DAPI.

• 

  Flow Cytometry (Intracellular) - Anti-BRCA1 antibody [MS13] (ab16781)

  Overlay histogram showing MCF7 cells stained with ab16781 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16781, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-BRCA1 antibody [MS13] (ab16781)This image is courtesy of an Abreview submitted by Dr Kirk McManus

  ab16781 (1/200) detecting BRCA1 in HeLa cells in conjunction with a goat anti-mouse secondary antibody conjugated to Cy3 (red). Cells were also counterstained with DAPI in order to highlight the nucleus (blue), treated with Bleomycin and incubated with an antibody against Histone H2AX in order to create and expose DNA double strand breaks (green). Please refer to abreviews for further details.

  See Abreview