Anti-APC antibody [CC-1]

• 产品名称

  Anti-APC抗体[CC-1]
  参阅全部 APC 一抗

• 描述

  小鼠单克隆抗体[CC-1] to APC

• 宿主

  Mouse

• 经测试应用

  适用于: Flow Cyt, IHC-P, ICC/IFmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Synthetic peptide corresponding to APC aa 1-226.

• 表位

  Epitope is within amino acid residues 1 - 226 of APC.

• 阳性对照

• Purkinje cells or cerebellum tissue.

• 常规说明

  APC is well suited for immunohistochemical and immunofluorescence studies of oligodendrocytes and optic nerves due to the antibody’s staining of the cell body as opposed to the myelinated processes.

  Although we list several publications demonstrating mouse reactivity, we have also received conflicting data from researchers demonstrating a lack of mouse reactivity. Therefore, we have removed mouse from our list of reactive species and no longer guarantee the product for this species.

  This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

   

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot. Store at +4°C.

• 存储溶液

  Preservative: 0.05% Sodium azide
  Constituents: 0.8766% Sodium chloride, 1.902% Tris glycine

• 纯度

  Protein G purified

• Primary antibody说明

  APC is well suited for immunohistochemical and immunofluorescence studies of oligodendrocytes and optic nerves due to the antibody’s staining of the cell body as opposed to the myelinated processes.

• 克隆

  单克隆

• 克隆编号

  CC-1

• 同种型

  IgG2b

The Abpromise guarantee

Abpromise™承诺保证使用ab16794于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 324 Human

• Omim: 611731 Human

• SwissProt: P25054 Human

• Unigene: 158932 Human

• 别名

• APC_HUMAN antibody

• CC1 antibody

• Deleted in polyposis 2.5 antibody

• DP2 antibody

• DP2.5 antibody

• DP3 antibody

• FAP antibody

• FPC antibody

• GS antibody

• Protein APC antibody

• Adenomatous Polyposis Coli antibody

• Adenomatous polyposis coli protein antibody

• Apc antibody

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APC antibody [CC-1] (ab16794)

  IHC analysis of formalin-fixed, paraffin embedded human cerebellum with ab16794.

  Antibody was used at a concentration of 2.5 µg/ml and sections were pretreated with heat using a pressure cooker prior to staining. Detection with DAB and hematoxylin as a counterstain.

  This image was generated using the ascites version of the product.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-APC antibody [CC-1] (ab16794)

  ICC/IF image of ab16794 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16794, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  This image was generated using the ascites version of the product.

• 

  Flow Cytometry - Anti-APC antibody [CC-1] (ab16794)

  Overlay histogram showing HCT116 cells stained with ab16794 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16794, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HCT116 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  This image was generated using the ascites version of the product.