Anti-F4/80 antibody [BM8]

• 产品名称

  Anti-F4/80抗体[BM8]
  参阅全部 F4/80 一抗

• 描述

  大鼠单克隆抗体[BM8] to F4/80

• 宿主

  Rat

• 特异性

  The monoclonal antibody BM8 recognizes a 125 kDa extracellular macrophage membrane molecule, highly restricted to mature macrophage subpopulations residing in tissue. This antibody does not cross react with any of the following cell types from Mouse: granulocytes, mast cells, platelets, lymphocytes, fibroblasts or endothelial cells.

• 经测试应用

  适用于: ICC, Flow Cyt, IHC-Frmore details

• 种属反应性

  与反应: Mouse, Human
  

• 免疫原

  Tissue, cells or virus corresponding to Mouse F4/80. BALB/c macrophages obtained from 14-day-old bone marrow cell cultures

• 阳性对照

• Flow Cyt: RAW and HeLa cells. IHC-Fr: Mouse liver and spleen tissues. ICC: Mouse brain and RAW246.7 cells.

• 常规说明

  ab16911 is the only macrophage marker that is able to distinguish non-destructive from destructive inflammation prcoesses in the pancreas. Furthermore it is a unique histological marker of the progression from peri-insulitis to beta-cell and diabetes in a mouse diabetes model.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

• 存储溶液

  Preservative: 0.02% Sodium azide
  Constituents: PBS, 0.1% BSA

• 浓度

• 100 µg 浓度为 0.1 mg/ml

• 纯度

  Protein G purified

• 纯化说明

  Provided as a 0.2µm filtered antibody solution.

• Primary antibody说明

  ab16911 is the only macrophage marker that is able to distinguish non-destructive from destructive inflammation prcoesses in the pancreas. Furthermore it is a unique histological marker of the progression from peri-insulitis to beta-cell and diabetes in a mouse diabetes model.

• 克隆

  单克隆

• 克隆编号

  BM8

• 同种型

  IgG2a

The Abpromise guarantee

Abpromise™承诺保证使用ab16911于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 2015 Human

• Entrez Gene: 13733 Mouse

• Omim: 600493 Human

• SwissProt: Q14246 Human

• SwissProt: Q61549 Mouse

• Unigene: 2375 Human

• Unigene: 2254 Mouse

• 别名

• AGRE1_HUMAN antibody

• Cell surface glycoprotein EMR1 antibody

• Cell surface glycoprotein F4/80 antibody

• DD7A5 7 antibody

• Egf like module containing mucin like hormone receptor like 1 antibody

• Egf like module containing mucin like hormone receptor like sequence 1 antibody

• EGF like module receptor 1 antibody

• EGF TM7 antibody

• EGF-like module receptor 1 antibody

• EGF-like module-containing mucin-like hormone receptor-like 1 antibody

• EGFTM7 antibody

• EMR 1 antibody

• EMR1 antibody

• EMR1 hormone receptor antibody

• Gpf480 antibody

• Ly71 antibody

• Lymphocyte antigen 71 antibody

• TM7LN3 antibody

• ADGRE1 antibody

• Adhesion G protein coupled receptor E1 antibody

• Adhesion G protein-coupled receptor E1 antibody

• 

  Flow Cytometry - Anti-F4/80 antibody [BM8] (ab16911)

  Detection of F4/80 in RAW cells. Red, black and blue line represent the isotype control, cells only and ab16911 at 10 μg/ml, respectively.

• 

  Immunohistochemistry (Frozen sections) - Anti-F4/80 antibody [BM8] (ab16911)

  ab16911 staining F4/80 on macrophages in mouse liver tissue by Immunohistochemistry (Frozen sections).

• 

  Immunocytochemistry - Anti-F4/80 antibody [BM8] (ab16911)This image is courtesy of an anonymous Abreview

  ab16911 staining F4/80 in Mouse brain cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with acetone and blocked with 5% BSA for 1 hour at 20°C. Samples were incubated with primary antibody (1/250) for 16 hours at 4°C. An Alexa Fluor®568-conjugated Goat anti-rat IgG polyclonal (1/1000) was used as the secondary antibody.

  See Abreview

• 

  Flow Cytometry - Anti-F4/80 antibody [BM8] (ab16911)

  Overlay histogram showing HeLa cells stained with ab16911 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16911, 1/10 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rat IgG (Fc) (ab96971) at 1/250 dilution for 30 min at 22°C. Isotype control antibody (black line) was rat IgG2a [aRTK2758] (ab18450, 2µg/1x106 cells cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a significantly decreased signal in HeLa cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.
  
  Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.
  

• 

  Immunohistochemistry (Frozen sections) - Anti-F4/80 antibody [BM8] (ab16911)This image is courtesy of an Abreview submitted by Miss Silke Vorwald

  ab16911 staining mouse spleen tissue sections by immunohistochemistry (frozen sections). Sections were paraformaldehyde fixed without permeabilization and blocked in 1% serum for 10 minutes at 20°C. The primary antibody was used undiluted and incubated with sample for 16 hour at 20°C. A Biotin conjugated goat polyclonal to rat Ig, diluted 1/500 was used as the secondary antibody.

  See Abreview

• 

  Immunocytochemistry - Anti-F4/80 antibody [BM8] (ab16911)

  ab16911 stained RAW246.7 cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16911 at 1in50 dilution) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed (ab150165) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.