Anti-Src antibody [Clone 327]

• 产品名称

  Anti-Src抗体[Clone 327]
  参阅全部 Src 一抗

• 描述

  小鼠单克隆抗体[Clone 327] to Src

• 宿主

  Mouse

• 特异性

  Reacts with both v-Src and c-Src proteins.

• 经测试应用

  适用于: Flow Cyt, WBmore details
  不适用于: ICC/IF,IHC-Fr or IHC-P

• 种属反应性

  与反应: Human
  

• 免疫原

  Full length native protein (purified) corresponding to Src. Antibody generated by immunizing BALB/c mice with full length native protein (purified) and then fusing with P3X63 Ag8.653 myeloma cells.

• 表位

  Epitope is within the SH3 domain of either v-Src or c-Src. We have no evidence for ab16885 cross-reacting with other src family kinases (e.g.fyn).

• 阳性对照

• WB: MCF7, HeLa, A431 and NIH/3T3 cell lysates. Wild-type HAP1 cell lysate. Flow Cyt: SH-SY5Y cells.

• 常规说明

  May appear as a doublet due to phosphorylation. May be used to precipitate active Src which can then be used in a kinase reaction.

  This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C. Do Not Freeze.

• 存储溶液

  Preservative: 0.1% Sodium azide
  Constituents: 0.2% Gelatin, 0.82% Sodium phosphate

• 纯度

  Tissue culture supernatant

• 纯化说明

  Purified from TCS.

• Primary antibody说明

  May appear as a doublet due to phosphorylation. May be used to precipitate active Src which can then be used in a kinase reaction.

• 克隆

  单克隆

• 克隆编号

  Clone 327

• 骨髓瘤

  P3x63-Ag8.653

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab16885于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用说明

Is unsuitable for ICC/IF,IHC-Fr or IHC-P.

• 数据库链接

• Entrez Gene: 6714 Human

• Omim: 190090 Human

• SwissProt: P00523 Human

• SwissProt: P12931 Human

• Unigene: 195659 Human

• 形式

  This protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), FYN (P06241), YES1 (P07947), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.

• 别名

• c SRC antibody

• CDNA FLJ14219 fis clone NT2RP3003800 highly similar to Rattus norvegicus tyrosine protein kinase pp60 c src mRNA antibody

• cSrc antibody

• EC 2.7.10.2 antibody

• Neuronal CSRC tyrosine specific protein kinase antibody

• Neuronal proto-oncogene tyrosine-protein kinase Src antibody

• Neuronal SRC antibody

• Oncogene SRC antibody

• OTTHUMP00000174476 antibody

• OTTHUMP00000174477 antibody

• p60 Src antibody

• p60-Src antibody

• p60c-src antibody

• p60Src antibody

• pp60c src antibody

• pp60c-src antibody

• pp60csrc antibody

• Proto oncogene tyrosine protein kinase Src antibody

• Proto-oncogene c-Src antibody

• Proto-oncogene tyrosine-protein kinase Src antibody

• Protooncogene SRC antibody

• Protooncogene SRC Rous sarcoma antibody

• Src antibody

• SRC Oncogene antibody

• SRC proto oncogene non receptor tyrosine kinase antibody

• SRC_HUMAN antibody

• SRC1 antibody

• Tyrosine kinase pp60c src antibody

• Tyrosine protein kinase SRC 1 antibody

• Tyrosine protein kinase SRC1 antibody

• v src avian sarcoma (Schmidt Ruppin A2) viral oncogene homolog antibody

• V src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog (avian) antibody

• v src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog avian antibody

• ASV antibody

• Avian sarcoma virus antibody

• AW259666 antibody

• 

  Western blot - Anti-Src antibody [Clone 327] (ab16885)

  Lane 1: Wild-type HAP1 cell lysate (20 µg)
  Lane 2: Src knockout HAP1 cell lysate (20 µg)
  Lane 3: HeLa cell lysate (20 µg)
  Lane 4: A431 cell lysate (20 µg)
  Lanes 1 - 4: Merged signal (red and green). Green - ab16885 observed at 60 kDa. Red - loading control, ab8227, observed at 42 kDa.

  ab16885 was shown to specifically react with Src when Src knockout samples were used. Wild-type and Src knockout samples were subjected to SDS-PAGE. ab16885 and ab8227 (loading control to beta Actin) were diluted 2.5µg/ml and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  This image was generated using the ascites version of the product.

• 

  Flow Cytometry - Anti-Src antibody [Clone 327] (ab16885)

  Overlay histogram showing SH-SY5Y cells stained with ab16885 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16885, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  This image was generated using the ascites version of the product.

• 

  Western blot - Anti-Src antibody [Clone 327] (ab16885)

  Lane 1: Wild-type HAP1 cell lysate (20 µg)
  Lane 2: Src knockout HAP1 cell lysate (20 µg)
  Lane 3: HeLa cell lysate (20 µg)
  Lane 4: A431 cell lysate (20 µg)

  Lanes 1 - 4: Merged signal (red and green). Green - ab16885 observed at 60 kDa. Red - loading control, ab8245, observed at 37 kDa.

  This western blot image is a comparison between ab16885 and a competitor's top cited rabbit polyclonal antibody.

  This image was generated using the ascites version of the product.

• 

  Western blot - Anti-Src antibody [Clone 327] (ab16885)This image is courtesy of an anonymous Abreview

  All lanes : Anti-Src antibody [Clone 327] (ab16885) at 2.5 µg/ml
  
  Lane 1 : Whole cell lysate prepared from human MCF-7 cells (Untreated)
  Lane 2 : Whole cell lysate prepared from human MCF-7 cells (Treated with 10uM PP2)
  
  Secondary
  All lanes : HRP conjugated Donkey polyclonal to mouse Ig at 1/5000 dilution
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Predicted band size: 59.7 kDa
  
  
  Exposure time: 3 minutes
  
  
  

  This image was generated using the ascites version of the product.

  See Abreview

• 

  Western blot - Anti-Src antibody [Clone 327] (ab16885)This image is a courtesy of Anonymous Abreview

  Anti-Src antibody [Clone 327] (ab16885) at 1/100 dilution + Lysate prepared from mouse NIH 3T3 cell line at 10 µg
  
  Secondary
  HRP-conjugated sheep monoclonal to mouse IgG at 1/5000 dilution
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Predicted band size: 59.7 kDa
  Observed band size: 60 kDawhy is the actual band size different from the predicted?
  
  
  Exposure time: 12 minutes
  
  
  

  This image was generated using the ascites version of the product.

  See Abreview