Anti-PAX5 antibody

• 产品名称

  Anti-PAX5抗体
  参阅全部 PAX5 一抗

• 描述

  兔多克隆抗体to PAX5

• 宿主

  Rabbit

• 经测试应用

  适用于: IHC-P, IPmore details

• 种属反应性

  与反应: Human
  预测可用于: Mouse, Rat

• 免疫原

  Synthetic peptide within Human PAX5 aa 350 to the C-terminus (C terminal). The exact sequence is proprietary.
  Database link: Q02548

• 常规说明

  
  

  This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

• 存储溶液

  pH: 7.60
  Preservative: 0.1% Sodium azide
  Constituents: PBS, 1% BSA

• 纯度

  Immunogen affinity purified

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab15164于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 5079 Human

• Entrez Gene: 18507 Mouse

• Omim: 167414 Human

• SwissProt: Q02548 Human

• SwissProt: Q02650 Mouse

• Unigene: 654464 Human

• Unigene: 439659 Mouse

• 别名

• B cell specific transcription factor antibody

• B-cell-specific transcription factor antibody

• BSAP antibody

• EBB-1 antibody

• KLP antibody

• Paired box 5 antibody

• Paired box gene 5 (B cell lineage specific activator protein) antibody

• Paired box gene 5 (B cell lineage specific activator) antibody

• Paired box gene 5 antibody

• Paired box homeotic gene 5 antibody

• Paired box protein Pax 5 antibody

• Paired box protein Pax-5 antibody

• Paired domain gene 5 antibody

• PAX 5 antibody

• PAX5 antibody

• PAX5_HUMAN antibody

• Transcription factor PAX 5 antibody

• B cell lineage specific activator antibody

• B cell lineage specific activator protein antibody

• B cell specific activator protein antibody

• 

  Immunoprecipitation - Anti-PAX5 antibody (ab15164)

  PAX5 was immunoprecipitated using 0.5mg Ramos whole cell extract, 5µg of Rabbit polyclonal to PAX5 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

  The antibody was incubated under agitation with Protein G beads for 10min, Ramos whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

  Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab15164.

  Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

  Band: 42kDa; PAX5

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody (ab15164)

  ab15164 staining PAX5 in human tonsil by Immunohistochemistry (FFPE-sections).