Anti-COX2 / Cyclooxygenase 2抗体
参阅全部 COX2 / Cyclooxygenase 2 一抗
兔多克隆抗体to COX2 / Cyclooxygenase 2
Rabbit
适用于: WB, Sandwich ELISA, IHC-Pmore details
与反应: Mouse, Human
预测可用于: Rat, Syrian hamster
Synthetic peptide within Rat COX2/ Cyclooxygenase 2 aa 550 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P35355
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
浓度
500 µl 浓度为 1 mg/ml
1 ml 浓度为 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab15191于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (19) | Use at an assay dependent concentration. Predicted molecular weight: 69 kDa. |
Sandwich ELISA | Use a concentration of 0.5 µg/ml. For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab90345 as Capture. | |
IHC-P | (8) | 1/100. |
hide
Unigene: 292547 Mouse
Unigene: 44369 Rat
Entrez Gene: 5743 Human
Entrez Gene: 19225 Mouse
Omim: 600262 Human
SwissProt: P35354 Human
SwissProt: Q05769 Mouse
Unigene: 196384 Human
Cyclooxygenase 2 antibody
Cyclooxygenase 2b antibody
Cyclooxygenase antibody
Cyclooxygenase-2 antibody
Cyclooxygenase2 antibody
EC 1.14.99.1 antibody
fj02a10 antibody
Glucocorticoid-regulated inflammatory cyclooxygenase antibody
Glucocorticoid-regulated inflammatory Prostaglandin G/H synthase antibody
GRIPGHS antibody
hCox 2 antibody
Macrophage activation-associated marker protein P71/73 antibody
OTTHUMP00000033524 antibody
PES-2 antibody
PGG/HS antibody
PGH synthase 2 antibody
PGH2_HUMAN antibody
PGHS 2 antibody
PGHS-2 antibody
PGHS2 antibody
PHS 2 antibody
PHS II antibody
PHS2 antibody
Prostaglandin endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody
Prostaglandin endoperoxide synthase 2 antibody
Prostaglandin G/H synthase 2 antibody
Prostaglandin G/H synthase 2 precursor antibody
Prostaglandin G/H synthase and cyclooxygenase antibody
Prostaglandin G/H synthase antibody
Prostaglandin H2 synthase 2 antibody
prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody
Prostaglandin-endoperoxide synthase 2 antibody
PTGS2 antibody
ptgs2a antibody
TIS10 antibody
TIS10 protein antibody
unp1239 antibody
wu:fj02a10 antibody
COX 2 antibody
COX-2 antibody
COX2 antibody
Western blot - Anti-COX2 / Cyclooxygenase 2 antibody (ab15191)
All lanes : Anti-COX2 / Cyclooxygenase 2 antibody (ab15191) at 1 µg/ml
Lane 1 : Recombinant human COX2 / Cyclooxygenase 2 protein (Active) (ab58868) at 0.1 µg
Lane 2 : Recombinant human COX2 / Cyclooxygenase 2 protein (Active) (ab58868) at 0.01 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 69 kDa
Exposure time: 10 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX2 / Cyclooxygenase 2 antibody (ab15191)This image is courtesy of an Abreview submitted by Mr Carl Hobbs
ab15191 staining mouse brain sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citrate buffer prior to blocking with 1% BSA for 10 minutes at RT. The primary antibody was diluted 1/2000 and incubated with the sample for 2 hours. A biotinylated goat anti-rabbit IgG antibody, diluted 1/300, was used as the secondary. Sections were preblocked in an Avidin-Biotin kit to mask endogenous biotin
Sandwich ELISA - Anti-COX2 / Cyclooxygenase 2 antibody (ab15191)
Standard Curve for COX2 / Cyclooxygenase 2 (Analyte: ab58868) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [AS66] to COX2 / Cyclooxygenase 2 (ab90345) at 1ug/ml and Detector Antibody Rabbit polyclonal to COX2 / Cyclooxygenase 2 (ab15191) at 0.5ug/ml
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX2 / Cyclooxygenase 2 antibody (ab15191)
ab15191 staining COX2 / Cyclooxygenase in human breast carcinoma by Immunohistochemistry (FFPE-sections).