Anti-Dystrophin antibody

• 产品名称

  Anti-Dystrophin抗体
  参阅全部 Dystrophin 一抗

• 描述

  兔多克隆抗体to Dystrophin

• 宿主

  Rabbit

• 经测试应用

  适用于: IHC-Fr, IHC-Pmore details

• 种属反应性

  与反应: Mouse, Human
  预测可用于: Rat, Dog, Pig

• 免疫原

  Synthetic peptide within Human Dystrophin aa 3650 to the C-terminus (C terminal). The exact sequence is proprietary.
  Database link: P11532

• 常规说明

  
  

  This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

• 存储溶液

  pH: 7.60
  Preservative: 0.1% Sodium azide
  Constituents: PBS, 1% BSA

• 浓度

• 批次浓度范围 100 µl 浓度为 0.2 - 0.24 mg/ml

• 批次浓度范围 1 ml 浓度为 0.2 - 0.24 mg/ml

• 批次浓度范围 500 µl 浓度为 0.2 - 0.52 mg/ml

• 纯度

  Immunogen affinity purified

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab15277于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

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• SwissProt: P11530 Rat

• Unigene: 495912 Human

• Unigene: 275608 Mouse

• Unigene: 416750 Mouse

• Unigene: 10307 Rat

• Entrez Gene: 1756 Human

• Entrez Gene: 13405 Mouse

• Entrez Gene: 24907 Rat

• NCBI: NP_004002.2 Human

• Omim: 300377 Human

• SwissProt: O97592 Dog

• SwissProt: P11532 Human

• SwissProt: P11531 Mouse

• 别名

• DMD_HUMAN antibody

• Duchenne muscular dystrophy protein antibody

• Dystrophin antibody

• Muscular dystrophy Duchenne and Becker types antibody

• BMD antibody

• CMD3B antibody

• DMD antibody

• 

  Immunohistochemistry (Frozen sections) - Anti-Dystrophin antibody (ab15277)

  Muscle stem cells (from normal mouse) were injected into the gastric muscle of an MDX mouse. Dystrophin staining: primary antibody ab15277 and secondary antibody is donkey anti-rabbit Alexa 594.

  This image was kindly supplied as part of the review submitted by Jessica Tebbets.

• 

  Immunohistochemistry (Frozen sections) - Anti-Dystrophin antibody (ab15277)Zhao C. et al PLoS One. 2014 Apr 29;9(4):e96279. doi: 10.1371/journal.pone.0096279. eCollection 2014.

  Immunofluorescence staining of dystrophin in W9, W987, and ESC. Myosin heavy chain (MHC) identified mouse muscle cells after differentiation. DAPI was used to stain nuclei.

  Seventy-two hours before engraftment, 8 week-old mdx/SCID mice received 14 Gy of irradiation localized to the hind limb muscles. On the day of engraftment, SM/C-2.6-positive myogenic cells were purified by fluorescence-activated cell sorting (FACS), using a BD Aria II FACS machine and the same labeling protocol as described above for FC analysis, resuspended in 30 µl of phosphate buffered saline (PBS), loaded into an insulin syringe (BD), and injected into the left tibialis anterior (TA) muscle of anesthetized mice. 7.5×105 differentiated and sorted W987 cells were injected. Control mice were injected with PBS alone. Three weeks following engraftment, TA muscles were harvested, fixed in 0.5% paraformaldehyde for 4 hours, dehydrated in 20% sucrose overnight and frozen in optimal cutting temperature (OCT) using liquid nitrogen cooled methyl-butane. Tissue blocks imbedded in OCT were cryosectioned and processed for immunocytochemical analysis using rabbit anti-dystrophin. Secondary antibodies used were donkey anti-rabbit conjugated to Alexafluor 594 and donkey anti-rat conjugated to Alexafluor 488 (Life Technologies). Nuclei were visualized using NucBlue Fixed Cell Stain (Life Technologies).

  Gene-corrected mdx iPSC W987, non-gene-corrected unexcised mdx iPSC W9 and wild-type ESC controls.

   

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody (ab15277)Sardone V. PLoS One. 2018 Mar 26;13(3):e0194540. doi: 10.1371/journal.pone.0194540. eCollection 2018. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

  Dystrophin quantification in a population of myofibres identified in entire muscle sections performing the double labelling anti-dystrophin ab15277 (red; 1/200 dilution) and anti-spectrin (green; 1/20 dilution).

  All the labellings were performed at RT. Human Muscle sections were incubated with the primary antibody combination (anti-dystrophin ab15277 and anti-spectrin) for 1 hour. After three washes with PBS sections were incubated with Alexa Fluor 488 conjugated anti-mouse IgG (1:100, Thermo Fisher Scientific, Hemel Hempstead, UK) and anti-rabbit biotinylated IgG (1:200; GE Healthcare, Amersham Pl, UK) for 30 minutes. PBS washes were performed and sections were incubated with Alexa Fluor 594 streptavidin conjugate (1:1000, Thermo Fisher Scientific, Hemel Hempstead, UK).

  Representative images of entire muscle sections stained and acquired by the Axio Scan slide scanner and processed with Definens algorithm derived from a control (a) and from a DMD patient (b).

  DMD: Duchenne Muscular Dystrophy.

   

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody (ab15277)

  Immunohistochemical staining of human skeletal muscle with ab15277.