Anti-Wnt1 antibody

• 产品名称

  Anti-Wnt1抗体
  参阅全部 Wnt1 一抗

• 描述

  兔多克隆抗体to Wnt1

• 宿主

  Rabbit

• 经测试应用

  适用于: ICC/IF, IHC-P, WBmore details

• 种属反应性

  与反应: Mouse, Human
  预测可用于: Rat

• 免疫原

  Synthetic peptide within Human Wnt1 aa 50-150 (N terminal). The exact sequence is proprietary.
  Database link: P04628

• 常规说明

  
  

  This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

• 存储溶液

  pH: 7.60
  Preservative: 0.1% Sodium azide
  Constituents: PBS, 1% BSA

• 纯度

  Immunogen affinity purified

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab15251于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 7471 Human

• Entrez Gene: 22408 Mouse

• Entrez Gene: 24881 Rat

• Omim: 164820 Human

• SwissProt: P04628 Human

• SwissProt: P04426 Mouse

• Unigene: 248164 Human

• Unigene: 1123 Mouse

• 别名

• oncogene Int1 antibody

• Proto oncogene protein Wnt 1 antibody

• Proto-oncogene Int-1 homolog antibody

• Proto-oncogene Wnt-1 antibody

• Wingless type MMTV integration site family member 1 antibody

• wingless-type MMTV integration site family, member 1 (oncogene INT1) antibody

• Wnt 1 antibody

• wnt1 antibody

• WNT1_HUMAN antibody

• BMND16 antibody

• INT1 antibody

• OI15 antibody

• 

  Western blot - Anti-Wnt1 antibody (ab15251)

  Anti-Wnt1 antibody (ab15251) at 1/25 dilution + NIH3T3 cell lysate
  
  Predicted band size: 41 kDa
  Observed band size: 41 kDa
  
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt1 antibody (ab15251)

  ab15251 staining Wnt1 in breast carcinoma by Immunohistochemistry (FFPE-sections).

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Wnt1 antibody (ab15251)

  ICC/IF image of ab15251 stained mouse 3T3 cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab15251, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt1 antibody (ab15251)

  Breast carcinoma cells stained with ab15251 (Immunohistochemistry, paraffin sections);