Anti-Glucose Transporter GLUT1抗体
参阅全部 Glucose Transporter GLUT1 一抗
兔多克隆抗体to Glucose Transporter GLUT1
Rabbit
适用于: ICC/IF, IHC-Pmore details
与反应: Human
预测可用于: Rat
Synthetic peptide within Human Glucose Transporter GLUT1 aa 450 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P11166
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7.40
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
浓度
批次浓度范围 100 µl 浓度为 0.15 - 0.24 mg/ml
批次浓度范围 500 µl 浓度为 0.24 - 0.3 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab15309于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (2) | Use at an assay dependent concentration. See Abreview. |
| IHC-P | (5) | 1/200. |
Entrez Gene: 6513 Human
Omim: 138140 Human
SwissProt: P11166 Human
Unigene: 473721 Human
Unigene: 721551 Human
Unigene: 3205 Rat
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody (ab15309)This image is courtesy of an Abreview submitted by Heiko Locher
ab15309 staining Glucose Transporter GLUT1 (green) in Human red blood cells tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 30 minutes at room temperature; antigen retrieval was by heat mediation in a citrate buffer, pH 6.0. Samples were incubated with primary antibody (1/500 in PBS-T + 1% PBS) for 12 hours. An Alexa Fluor® 488-conjugated Donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody. Red - autofluorescence of erythrocytes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody (ab15309)
ab15309 staining Glucose Transporter GLUT1 in human esophagous by Immunohistochemistry (FFPE-sections).
Immunocytochemistry/ Immunofluorescence - Anti-Glucose Transporter GLUT1 antibody (ab15309)
ab15309 at a 1/100 dilution staining rat cells (neural stem cells from adult subventricular zone) by Immunocytochemistry/Immunofluorescence. The cells were incubated with the antibody for 18 hours and then bound antibody was detected using a Cy3 conjugated Goat anti-rabbit IgG (H + L).
This image is courtesy of an Abreview submitted by Martin Maurer.
Immunocytochemistry/ Immunofluorescence - Anti-Glucose Transporter GLUT1 antibody (ab15309)
ICC/IF image of ab15309 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15309, 1µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
