Anti-Presenilin 1/PS-1 antibody [APS 18]

• 产品名称

  Anti-Presenilin 1/PS-1抗体[APS 18]
  参阅全部 Presenilin 1/PS-1 一抗

• 描述

  小鼠单克隆抗体[APS 18] to Presenilin 1/PS-1

• 宿主

  Mouse

• 经测试应用

  适用于: WB, IHC-P, ICC/IFmore details

• 种属反应性

  与反应: Mouse, Human
  预测可用于: Cynomolgus monkey

• 免疫原

  Synthetic peptide corresponding to Human Presenilin 1/PS-1 aa 300-400.
  Database link: P49768

  Run BLAST with Run BLAST with 

• 阳性对照

• WB: T-47D, MCF7, Daudi, SH-SY5Y, Caco-2 cell lysate. IHC-P: Human liver and tonsil tissue. ICC: MCF-7 cells, A2058 melanoma cells, mouse fibroblasts and HeLa cells.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.05% Sodium azide
  Constituents: 99% PBS, 0.1% BSA

• 浓度

• 200 µg 浓度为 1 mg/ml

• 纯度

  Protein G purified

• 克隆

  单克隆

• 克隆编号

  APS 18

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab15458于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 5663 Human

• Entrez Gene: 19164 Mouse

• Omim: 104311 Human

• SwissProt: P49768 Human

• SwissProt: P49769 Mouse

• Unigene: 3260 Human

• Unigene: 998 Mouse

• 别名

• Homo Sapiens Clone CC44 Senilin 1 antibody

• Presenilin-1 CTF12 antibody

• Protein S182 antibody

• PS 1 antibody

• PS-1 antibody

• PS1-CTF12 antibody

• PSEN1 antibody

• PSN1_HUMAN antibody

• PSNL1 antibody

• S182 antibody

• AD3 antibody

• Ad3h antibody

• FAD antibody

• 

  Western blot - Anti-Presenilin 1/PS-1 antibody [APS 18] (ab15458)

  All lanes : Anti-Presenilin 1/PS-1 antibody [APS 18] (ab15458) at 1/500 dilution
  
  Lane 1 : T-47D (human ductal breast epithelial tumor cell line) whole cell lysate
  Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
  Lane 3 : Daudi (human Burkitt's lymphoma cell line) whole cell lysate
  Lane 4 : SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate
  Lane 5 : Caco-2 (human colorectal adenocarcinoma cell line) whole cell lysate
  
  Lysates/proteins at 30 µg per lane.
  
  Secondary
  All lanes : Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP at 1/4000 dilution
  
  Predicted band size: 53 kDa
  
  

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Presenilin 1/PS-1 antibody [APS 18] (ab15458)

  Immunofluorescent analysis of Presenilin 1 / PS-1 using Presenilin 1 / PS-1 Monoclonal antibody (APS 18) ab115458 shows staining in MCF-7 cells. Presenilin 1 / PS-1 staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Presenilin 1 / PS-1 ab115458 at a dilution of 1:20-1:100 over night at 4 ?C washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
  
  

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Presenilin 1/PS-1 antibody [APS 18] (ab15458)

  Immunofluorescent analysis of Presenilin 1 / PS-1 using Presenilin 1 / PS-1 Monoclonal antibody (APS 18) ab115458 shows staining in A2058 melanoma cells. Presenilin 1 / PS-1 staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Presenilin 1 / PS-1 ab115458 at a dilution of 1:20-1:100 over night at 4 ?C washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
  
  

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Presenilin 1/PS-1 antibody [APS 18] (ab15458)

  Immunofluorescent analysis of Presenilin 1 / PS-1 using Presenilin 1 / PS-1 Monoclonal antibody (APS 18) ab115458 shows staining in HeLa cells. Presenilin 1 / PS-1 staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Presenilin 1 / PS-1 ab115458 at a dilution of 1:20-1:100 over night at 4 ?C washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
  
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Presenilin 1/PS-1 antibody [APS 18] (ab15458)

  Immunohistochemistry was performed on normal biopsies of deparaffinized Human liver tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Presenilin 1 / PS-1 ab15458 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Presenilin 1/PS-1 antibody [APS 18] (ab15458)

  Immunohistochemistry was performed on normal biopsies of deparaffinized Human tonsil tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal antibody recognizing Presenilin 1 / PS-1 ab15458 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  
  

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Presenilin 1/PS-1 antibody [APS 18] (ab15458)

  IF showing PS1 in mouse fibroblasts using ab15458.