Anti-Claudin 4抗体
参阅全部 Claudin 4 一抗
兔多克隆抗体to Claudin 4
Rabbit
适用于: WB, IHC-Pmore details
与反应: Human
预测可用于: Mouse, Pig
Synthetic peptide within Human Claudin 4 aa 150 to the C-terminus. The exact sequence is proprietary.
Database link: O14493
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
浓度
批次浓度范围 500 µl 浓度为 0.8 - 2 mg/ml
1 ml 浓度为 1 mg/ml
批次浓度范围 100 µl 浓度为 1 - 2 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab15104于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) | Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 22 kDa). |
| IHC-P | (2) | 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Entrez Gene: 1364 Human
Entrez Gene: 12740 Mouse
Omim: 602909 Human
SwissProt: O14493 Human
SwissProt: O35054 Mouse
Unigene: 647036 Human
Unigene: 699253 Human
Unigene: 7339 Mouse
CLDN 4 antibody
CLDN4 antibody
Clostridium perfringens enterotoxin receptor 1 antibody
Clostridium perfringens enterotoxin receptor antibody
CPE R antibody
CPE receptor antibody
CPE-R antibody
CPE-receptor antibody
CPER antibody
CPETR 1 antibody
CPETR antibody
CPETR1 antibody
hCPE R antibody
WBSCR8 antibody
Williams Beuren syndrome chromosome region 8 protein antibody
Williams-Beuren syndrome chromosomal region 8 protein antibody
Claudin-4 antibody
Claudin4 antibody
CLD4_HUMAN antibody
Western blot - Anti-Claudin 4 antibody (ab15104)
All lanes : Anti-Claudin 4 antibody (ab15104) at 1 µg/ml
Lane 1 : Wild-type MCF7 cell lysate
Lane 2 : CLDN4 knockout MCF7 cell lysate
Lane 3 : PC-3 cell lysate
Lane 4 : U-2 OS cell lysate
Lane 5 : LNCaP cell lysate
Lane 6 : SW480 cell lysate
Lane 7 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 17 kDawhy is the actual band size different from the predicted?
False colour image of Western blot: Anti-Claudin 4 antibody staining at 1 ug/ml, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab15104 was shown to bind specifically to Claudin 4. A band was observed at 17 kDa in wild-type MCF7 cell lysates with no signal observed at this size in CLDN4 knockout cell line ab274946 (knockout cell lysate ab275004). To generate this image, wild-type and CLDN4 knockout MCF7 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 4 antibody (ab15104)Lean QY et al. PLoS One (2015) Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Immunohistochemical analysis of healthy and colitis mouse colon sections (untreated and treated with enoxaparin) labeling claudin-4 with ab15104 at 1/200. ab7090, anti-rabbit immunoglobulin G conjugated to horseradish peroxidase (HRP) at 1/300 was used as the secondary antibody. ab64238 at 1/50 was used to develop the histological signal.
Antigen retrieval was performed by incubating the sections for 10 minutes at 97°C in 1 mM EDTA buffer, pH 8.0 or 10 mM citrate buffer, pH 6.0.
Control, C; untreated colitis, DSS; oral enoxaparin, OE; intraperitoneal injection of enoxaparin, IPE.
Western blot - Anti-Claudin 4 antibody (ab15104)
Anti-Claudin 4 antibody (ab15104) at 1 µg/ml + SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 20 kDawhy is the actual band size different from the predicted?
Additional bands at: 125 kDa, 34 kDa, 35 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 4 antibody (ab15104)
Formalin fixed paraffin embedded human tonsil stained with ab15104 (1/200, 10 min at room temperature).
