Anti-E Cadherin抗体- Intercellular Junction Marker
参阅全部 E Cadherin 一抗
兔多克隆抗体to E Cadherin - Intercellular Junction Marker
Rabbit
适用于: IHC-Pmore details
与反应: Human
预测可用于: Pig
Recombinant fragment within Human E Cadherin aa 600-750. The exact sequence is proprietary.
Database link: P12830
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
浓度
500 µl 浓度为 0.03 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab15148于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (2) | 1/30. for 10 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. |
Entrez Gene: 999 Human
Omim: 192090 Human
SwissProt: P12830 Human
Unigene: 461086 Human
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody - Intercellular Junction Marker (ab15148)This image is courtesy of an anonymous Abreview
ab15148 staining E Cadherin in Pig Cervix uteri tissue sections by IHC-P (Formaldehyde-fixed, Paraffin-embedded sections). Tissue samples were fixed with formaldehyde and blocked with 10% goat serum for 1 hour at 37°C; antigen retrieval was by heat mediation in 10mM citrate at pH 6 for 2 minutes. The sample was incubated with primary antibody (1/50) at 4°C for 12 hours. An HRP-conjugated goat polyclonal to rabbit IgG (undiluted) was used as secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody - Intercellular Junction Marker (ab15148)
Immunohistochemical staining of formalin fixed paraffin embedded human skin using ab15148.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody - Intercellular Junction Marker (ab15148)This image is a courtesy of Anonymous Abreview
ab15148 staining E Cadherin in human stomach tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before heat mediated antigen retrieval in Citrate pH 6.0 and then blocking with 5% serum for 1 hour at 23°C was performed. The primary antibody was used diluted 1/50 and incubated with sample for 1 hour at 23°C. A HRP conjugated goat polyclonal to rabbit IgG was used undiluted as secondary antibody.
