Anti-14-3-3 beta抗体
参阅全部 14-3-3 beta 一抗
兔多克隆抗体to 14-3-3 beta
Rabbit
适用于: ICC/IF, WB, IHC-Pmore details
与反应: Human
预测可用于: Mouse, Rat
Synthetic peptide within Human 14-3-3 beta aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: P31946
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
浓度
500 µl 浓度为 0.01 mg/ml
1 ml 浓度为 0.01 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab15260于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | Use a concentration of 5 µg/ml. | |
| WB | (3) | Use at an assay dependent concentration. Predicted molecular weight: 28 kDa. |
| IHC-P | 1/100. |
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Unigene: 34319 Mouse
Unigene: 485025 Mouse
Unigene: 8653 Rat
Entrez Gene: 7529 Human
Entrez Gene: 54401 Mouse
Omim: 601289 Human
SwissProt: P31946 Human
SwissProt: Q9CQV8 Mouse
Unigene: 643544 Human
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Western blot - Anti-14-3-3 beta antibody (ab15260)
Anti-14-3-3 beta antibody (ab15260) at 1/25 dilution + HeLa cell lysate
Predicted band size: 28 kDa
Observed band size: 28 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 beta antibody (ab15260)
ab15260 staining 14-3-3 beta in human brain by Immunohistochemistry (FFPE-sections).
Immunocytochemistry/ Immunofluorescence - Anti-14-3-3 beta antibody (ab15260)
ICC/IF image of ab15260 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15260, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
